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Papers
61,005 resultsShowing papers similar to An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates
ClearAssessment of RNA extraction protocols from cladocerans
Researchers evaluated multiple RNA extraction protocols for use with cladocerans, small crustaceans important in freshwater ecotoxicology. Protocol performance varied significantly in yield and RNA quality across the species tested. Reliable RNA extraction is the foundation for gene expression studies that assess how organisms like water fleas respond to environmental stressors including plastic pollution.
Detection, counting and characterization of nanoplastics in marine bioindicators: a proof of principle study
Researchers demonstrated a proof-of-concept workflow for detecting and counting nanoplastic particles (below 1 µm) in marine invertebrate tissues using electron microscopy and spectroscopic confirmation, finding nanoplastics in marine bioindicator species and establishing a methodology for future monitoring programs.
Development and Validation of an Efficient Method for Processing Microplastics in Biota Samples
A new one-step laboratory method was developed to efficiently digest and extract microplastics from mussel and fish tissue samples. Standardized extraction methods are critical for making microplastic studies comparable across different labs and species.
A review of current approaches for the study of microplastic contamination in crustaceans
This review assessed current methodological approaches for studying microplastic contamination in crustaceans, evaluating techniques for extraction, identification, and quantification while highlighting gaps in standardization that limit cross-study comparisons and risk assessment for these ecologically important invertebrates.
Point-of-care testing for measuring haemolymph glucose in invertebrates is not a valid method
This study found that point-of-care blood glucose devices designed for humans are not reliable for measuring hemolymph glucose in invertebrates used in ecotoxicological studies. The findings have implications for the accuracy of field assessments of invertebrate health, including studies examining microplastic effects.
Isolation of microplastics in biota-rich seawater samples and marine organisms
Researchers developed and tested methods for extracting microplastics from seawater samples rich in biological material and from the tissues of marine organisms. They found that enzymatic digestion was the most effective and least destructive approach for isolating microplastics from biological samples, outperforming acid and alkaline treatments. The study provides improved laboratory protocols that will help scientists more accurately measure microplastic contamination in marine environments and wildlife.
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
This methods paper describes a low-maintenance lab culture system for the marine filter-feeder Oikopleura dioica, a zooplankton organism useful for studying microplastic ingestion. Reliable culturing of this species supports research into how filter-feeders interact with microplastics and other particles in the water column.
Flow cytometric analysis of hepatopancreatic cells from Armadillidium vulgare highlights terrestrial isopods as efficient environmental bioindicators in ex vivo settings
Researchers optimized a method for analyzing individual cells from the digestive organ of pill bugs (terrestrial isopods) and used it to detect pollution-related stress across sites with different contamination levels. The findings position these common soil invertebrates as practical, early-warning biological indicators for environmental pollution monitoring.
Efficiency assessment of microplastic extraction from green mussel \(\textit{Perna viridis}\) Linnaeus
This study evaluated and optimized laboratory extraction methods for isolating and identifying microplastics from the tissues of green mussels, which accumulate plastic particles through their filter-feeding habits. Reliable extraction methods are essential for accurately quantifying microplastic contamination in shellfish consumed by humans.
DEVELOPMENT OF A NOVEL PROTOCOL FOR THE EXTRACTION OF SMALL MICROPLASTICS (1-5 µm) FROM BIOLOGICAL TISSUES
Researchers developed a novel extraction protocol to isolate and quantify small microplastics (1–5 µm) from biological tissues, addressing a major gap in marine contamination studies. The method improves detection of these hard-to-analyze particles, which are more likely to penetrate cells and accumulate in organisms.
Optimization of Extraction Method for Microplastic from Invertebrates Using Pancreatic Enzymes
Researchers optimized a method for extracting microplastics from invertebrates using pancreatic enzymes, which digest biological tissue without degrading plastic polymers. Improved extraction methods are essential for accurately measuring microplastic contamination in organisms and for assessing exposure levels across food chains.
Microplastic extraction from digestive tracts of large decapods
This study developed and validated a method for extracting microplastics from the digestive tracts of large decapod crustaceans, enabling accurate quantification and polymer identification. The protocol minimizes contamination and is suitable for routine monitoring of seafood species.
Enzymatic digestion method development for long-term stored chitinaceous planktonic samples
Researchers developed an enzymatic digestion method for extracting microplastics from long-term preserved chitinaceous planktonic crustacean samples, comparing material from 1985 and 2020 North Sea collections. The method enables retrospective microplastic analysis in archived time-series biological collections, providing a tool for investigating historical changes in marine microplastic exposure.
Occurrence, Distribution, and Extraction Methods of Microplastics in Marine Organisms
This review synthesizes global data on microplastic occurrence and distribution across marine organisms, comparing the advantages and limitations of different extraction and identification methods used in the field.
Experimental development of a new protocol for extraction and characterization of microplastics in fish tissues: First observations in commercial species from Adriatic Sea
Researchers developed and tested a new protocol for the extraction and characterization of microplastics from environmental samples, optimizing steps for recovery efficiency and polymer identification accuracy.
Screening for microplastics in sediment, water, marine invertebrates and fish: Method development and microplastic accumulation
Researchers developed improved methods for extracting microplastics from biological samples and sediments, using enzymatic digestion that achieved 97% particle recovery without damaging the plastics. When applied to field samples from the North Sea and Swedish coast, they found microplastics in 8 of 9 invertebrate species tested and in 68% of brown trout. Mussel tissue contained roughly a thousand times more microplastic particles per kilogram than surrounding sediment or water.
Chitinase digestion for the analysis of microplastics in chitinaceous organisms using the terrestrial isopod Oniscus asellus L. as a model organism
Researchers developed and validated a chitinase digestion protocol for extracting microplastics from chitinaceous organisms, using the terrestrial isopod Oniscus asellus as a model, and compared four protocols for dissolving chitin-containing tissue without degrading microplastic particles.
Microplastic in wild populations of the omnivorous crab Carcinus aestuarii: A review and a regional-scale test of extraction methods, including microfibres
Researchers compared enzymatic and potassium hydroxide digestion methods for extracting microplastics from wild crab tissues, finding that KOH is faster and cheaper for small organisms while enzymatic digestion is preferable for larger samples requiring longer treatment, with actual microplastic prevalence in wild crabs at just 5.5% — all microfibres, predominantly polyester.
Extraction and identification of microplastics from mussels: Method development and preliminary results
Scientists developed and validated a method for extracting and identifying microplastics from mussel tissue, then applied it to measure contamination in commercially harvested mussels. The method produced reliable, reproducible results, providing a practical tool for monitoring microplastic levels in one of the world's most widely consumed shellfish.
Improvements in histological technique for the ecotoxicological assessment using small biological samples
Researchers improved histological techniques for ecotoxicological assessment of small biological samples, refining tissue processing and staining protocols to better characterize cellular morphology and physiology in small test organisms commonly used in bioassays.
Identifying a suitable digestion protocol for microplastic extraction from Isidella elongata (Esper, 1788): New insights for cold-water corals
Researchers identified a suitable tissue digestion protocol for extracting microplastics from Isidella tentaculum, a deep-sea coral, helping standardize methodologies for monitoring microplastic pollution in marine invertebrates where existing protocols are lacking.
Genes of filter-feeding species as a potential toolkit for monitoring microplastic impacts
Researchers developed a genetic toolkit using candidate genes from filter-feeding marine species to monitor the biological impacts of microplastic exposure in natural environments. They identified six genes across nine species that show measurable expression changes when organisms encounter microplastics. The study offers a practical molecular approach for tracking how microplastic pollution is actually affecting wild marine populations.
Improving the proteome coverage of Daphnia magna ‐ implications for future ecotoxicoproteomics studies
This study improved the method for identifying proteins in the water flea Daphnia magna, a key organism used in ecotoxicology research, by optimizing protein extraction. Better proteomic tools will support future studies on how microplastics and other contaminants affect this sentinel species at the molecular level.
Sampling, Isolating and Identifying Microplastics Ingested by Fish and Invertebrates *
This methodological review critically evaluated sampling, isolation, and identification techniques for microplastics ingested by fish and invertebrates, identifying common sources of error including contamination during processing, particle loss, and misidentification — and recommending standardized protocols.