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Papers
20 resultsShowing papers similar to In vivo super-resolution of the brain – How to visualize the hidden nanoplasticity?
ClearImaging dendritic spines in the hippocampus of a living mouse by 3D-STED microscopy
Researchers extended 3D STED super-resolution microscopy to image dendritic spines in the hippocampus of living mice, achieving nanoscale resolution in three dimensions within deep brain tissue and opening new possibilities for studying synaptic structures in vivo.
Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy
This paper is not about microplastics; it presents an in vivo 3D super-resolution microscopy methodology for imaging dendritic spines in the mouse hippocampus.
Single-Particle Resolution Fluorescence Microscopy of Nanoplastics
Researchers developed a super-resolution fluorescence microscopy technique that enables single-particle detection and precise localization of nanoplastics in biological tissues and environmental samples. This advancement addresses a major limitation in nanoplastic research, as conventional microscopy lacks the resolution to distinguish individual nanoplastics from background fluorescence or free dye.
Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture revealed by STED nanoscopy
This neuroscience study used STED super-resolution microscopy to show that environmental enrichment enhances the size and structural complexity of synapses in the brain. It is a basic neuroscience paper not related to microplastics or environmental plastic pollution.
Editorial: 15 years of Frontiers in Cellular Neuroscience: super-resolution microscopy in the healthy and the injured brain
This editorial introduces a research collection on super-resolution microscopy techniques applied to the healthy and injured brain, highlighting how methods that surpass the diffraction limit of classical fluorescence microscopy are revealing new insights into synaptic organization and cellular pathology. The collection covers advances in both imaging hardware and computational image analysis relevant to neuroscience research.
Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy
Researchers used STED nanoscopy to reveal that environmental enrichment enhances the patterning and remodeling of synaptic nanoarchitecture in the brain, demonstrating experience-dependent structural plasticity at an unprecedented nanoscale resolution.
Cortex-Wide, Cellular-Resolution Volumetric Imaging with a Modular Two-Photon Imaging Platform
This paper presents Meso2P, a new two-photon microscope capable of imaging the entire mouse cortex at single-cell resolution — a significant advance in neuroscience instrumentation. Among its demonstrated applications is the ability to track the distribution of micro- and nanoplastic particles in living brain tissue in real time. While primarily a neuroscience tool, its capacity to visualize nanoplastics in the brain non-invasively could become valuable for directly studying how plastic particles move through and accumulate in neural tissue.
Stable but not rigid: Chronic in vivo STED nanoscopy reveals extensive remodeling of spines, indicating multiple drivers of plasticity
Researchers used chronic in vivo STED nanoscopy to track dendritic spine geometry in mouse neocortex over one month, finding that spine heads and necks undergo extensive, largely uncorrelated remodeling even without induced plasticity. The results indicate that multiple independent mechanisms drive spine structural dynamics beyond LTP-dependent pathways.
Single particle-resolution fluorescence microscopy of nanoplastics
Researchers developed a fluorescence microscopy technique capable of imaging and identifying individual nanoplastic particles. The method enables single-particle resolution detection of nanoplastics, which is a key step toward better quantifying these otherwise invisible particles in environmental samples.
Fluorescence radial fluctuation enables two-photon super-resolution microscopy
Researchers applied super-resolution radial fluctuation analysis to two-photon microscopy to achieve high-resolution imaging deep within brain tissue. The technique achieved spatial resolution comparable to structured illumination microscopy at depths of several hundred micrometers and enabled the first in vivo super-resolution imaging of the fifth layer of the cerebral cortex, offering an accessible upgrade for existing two-photon microscope systems.
Author response: Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy
Researchers developed a virtually crosstalk-free two-color in vivo STED nanoscopy system to simultaneously superresolve PSD95 post-synaptic density dynamics and spine geometry in the mouse cortex, finding that environmental enrichment enhanced the patterning and remodeling of synaptic nanoarchitecture in ways not detectable by conventional microscopy.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Whole-Tissue Distribution Analysis for Visualization of Nanoplastics in the Mouse Brain
Researchers used whole-tissue clearing combined with fluorescence microscopy to visualize the three-dimensional distribution of nanoplastics throughout intact mouse brains without sectioning. This approach revealed nanoplastic accumulation patterns across brain regions that section-based imaging would have missed, demonstrating a valuable method for mapping nanoplastic biodistribution in structurally complex organs.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
Super-resolution imaging of micro- and nanoplastics using confocal Raman with Gaussian surface fitting and deconvolution
Researchers used confocal Raman imaging with Gaussian surface fitting to achieve super-resolution visualization of micro- and nanoplastics beyond the optical diffraction limit, enabling identification and imaging of nanoplastic particles smaller than conventional Raman microscopy can resolve.
Editor's evaluation: Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy
This is an editorial evaluation of a neuroscience study on how environmental enrichment changes the physical structure of synaptic connections in the brain. It is not related to microplastics or environmental pollution.
Far-field super-resolution chemical microscopy
Researchers reviewed recent advances in "far-field chemical microscopy," a group of techniques that create detailed molecular maps of materials without needing dyes or labels, while also breaking the traditional limits of optical resolution. These super-resolution chemical imaging methods are opening new windows for studying biological systems, identifying environmental contaminants like microplastics, and inspecting materials at the nanoscale.
A quantitative study of nanoplastics within cells using magnetic resonance imaging
Researchers developed a magnetic resonance imaging strategy to quantify nanoplastics internalized by mouse macrophage cells, providing a novel non-invasive approach for tracking nanoplastic uptake and distribution within living organisms.
White matter hyperintensities and microplastics
Researchers aligned ante-mortem and post-mortem brain MRI scans and found large amounts of plastic particles in brain regions showing white matter hyperintensities, which are associated with small vessel disease. Using a novel optical imaging approach, they identified the cellular locations of these plastics in areas with vascular injury and amyloid plaques. The study raises important questions about whether microplastics in the brain contribute to or result from pre-existing vascular damage in people with cognitive impairment.
Super-resolution Raman imaging towards visualisation of nanoplastics
Super-resolution Raman imaging was evaluated as a method to visualize nanoplastics smaller than the conventional diffraction-limited laser spot size, overcoming a key barrier in nanoplastic characterization. The technique extends confocal Raman capabilities into the nanoscale detection range needed for environmental nanoplastic analysis.