Papers

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Article Tier 2

Role of extracellular polymeric substances in the acute inhibition of activated sludge by polystyrene nanoparticles

Researchers investigated how extracellular polymeric substances — the sticky biofilm matrix produced by bacteria — affected the acute inhibition of activated sludge by microplastics, finding that these substances played a protective role by reducing microplastic toxicity in wastewater treatment systems.

2018 Environmental Pollution 157 citations
Article Tier 2

Impact of Polyethylene Terephthalate Microplastics on Aerobic Granular Sludge Structure and EPS Composition in Wastewater Treatment

Researchers investigated how PET microplastics affect the structure and function of aerobic granular sludge used in wastewater treatment. Higher microplastic concentrations led to changes in granule size, altered the composition of extracellular polymeric substances, and shifted microbial community structure. The findings suggest that microplastic contamination in wastewater could compromise the stability and efficiency of biological treatment processes.

2025 Water 6 citations
Article Tier 2

Response of aerobic granular sludge under polyethylene microplastics stress: Physicochemical properties, decontamination performance, and microbial community

Researchers investigated the impact of polyethylene microplastics on aerobic granular sludge used in wastewater treatment. The study found that microplastics significantly disrupted sludge structure, settling properties, and enzyme activities related to denitrification and phosphorus removal, with increased reactive oxygen species and cell membrane damage at higher concentrations.

2022 Journal of Environmental Management 79 citations
Article Tier 2

Impact of the concentration and type of microplastics on the treatment efficiency and biomass structure in aerobic granular sludge reactors

Researchers investigated how polyethylene terephthalate and polyethylene microplastics at varying concentrations affect treatment efficiency, granule morphology, extracellular polymer production, microbial species composition, and metabolic activity in aerobic granular sludge reactors used for wastewater treatment. The study tested four hypotheses regarding MP effects on both the biological performance and structural integrity of aerobic granular sludge as a promising technology for MP-contaminated wastewater.

2022 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Adsorption Characteristics between DifferentSizes of Microplastics and EPS Fractionsof Anaerobic Granular Sludge

This study examined how different sizes of polystyrene microplastics interact with extracellular polymeric substances in anaerobic granular sludge. Researchers found that smaller microplastics stimulated greater production of these substances, while larger particles had the opposite effect, suggesting particle size plays a key role in how microplastics affect wastewater treatment processes.

2024 Polish Journal of Environmental Studies 2 citations
Article Tier 2

Mechanisms underlying the detrimental impact of micro(nano)plastics on the stability of aerobic granular sludge: Interactions between micro(nano)plastics and extracellular polymeric substances

Researchers found that both micro- and nanoplastics at realistic concentrations harmed the performance of aerobic granular sludge, a technology used for wastewater treatment, by reducing its ability to remove nitrogen. The plastic particles interacted with the sticky substances that hold the sludge granules together, weakening their structural integrity. The study reveals a specific mechanism by which plastic pollution can undermine wastewater treatment systems that communities rely on for clean water.

2024 Journal of Hazardous Materials 31 citations
Article Tier 2

Revealing the influencing mechanisms of polystyrene microplastics (MPs) on the performance and stability of the algal-bacterial granular sludge

Researchers investigated how polystyrene microplastics affect algal-bacterial granular sludge used in wastewater treatment, finding that the sludge removed over 96% of incoming microplastics but that microplastics inhibited COD removal by 2.6 to 4.1% and total phosphorus removal by 2.9 to 5.8%. Structural stability was compromised through oxidative stress, altered protein composition, and reduced abundance of key functional bacteria.

2022 Bioresource Technology 73 citations
Article Tier 2

Impact of Nano–Sized Polyethylene Terephthalate on Microalgal–Bacterial Granular Sludge in Non–Aerated Wastewater Treatment

This study found that nano-sized PET microplastics at concentrations up to 30 mg/L had little impact on a microalgal-bacterial wastewater treatment system, but at 50 mg/L began degrading performance after two weeks by suppressing algal growth and disrupting energy metabolism in the microbial community. The granular sludge responded by producing extracellular polymers that adsorbed the nanoplastics, acting as a partial defense mechanism. These findings suggest that current nanoplastic contamination levels in municipal wastewater are unlikely to severely compromise this emerging treatment technology, but higher concentrations could be problematic.

2023 Water 6 citations
Article Tier 2

Adaptation responses of microalgal-bacterial granular sludge to polystyrene microplastic particles in municipal wastewater

Researchers found that polystyrene microplastics of varying sizes did not significantly impair organic, ammonia, or phosphorus removal in microalgal-bacterial granular sludge systems treating municipal wastewater, though the microplastics did alter microbial community composition.

2022 Environmental Science and Pollution Research 28 citations
Article Tier 2

Biopolymers in Aerobic Granular Sludge—Their Role in Wastewater Treatment and Possibilities of Re-Use in Line with Circular Economy

This review examines the composition and role of extracellular polymeric substances in aerobic granular sludge, finding that the high alginate content of granules not only improves wastewater treatment performance but also presents opportunities for polymer recovery aligned with circular economy principles.

2021 Energies 27 citations
Article Tier 2

Laboratory-scale study of a biodegradable microplastic polylactic acid stabilizing aerobic granular sludge system

Researchers found that adding biodegradable polylactic acid microplastics to aerobic granular sludge systems at low-to-moderate concentrations actually stabilized granule formation and improved pollutant removal by stimulating extracellular polymer secretion.

2022 Environmental Pollution 30 citations
Article Tier 2

Effects of exposure to polyether sulfone microplastic on the nitrifying process and microbial community structure in aerobic granular sludge

Scientists added polyether sulfone microplastics to aerobic granular sludge bioreactors at different concentrations and found only minor effects on ammonia removal but an increase in total nitrogen removal efficiency of 5.6%, along with shifts in nitrifying microbial community structure.

2020 Bioresource Technology 110 citations
Article Tier 2

Microplastic removal in aquatic systems using extracellular polymeric substances (EPS) of microalgae

Researchers tested whether extracellular polymeric substances produced by microalgae could remove microplastics from water. Among four microalgae strains tested under stress conditions, Spirulina produced the most polymeric substances and formed the largest aggregates with microplastic particles. The study suggests that microalgae-based bioremediation could offer a sustainable, low-cost approach to reducing microplastic contamination in water sources.

2025 Sustainable Environment 5 citations
Article Tier 2

Extracellular polymeric substances in green alga facilitate microplastic deposition

Extracellular polymeric substances secreted by the green alga Spirogyra facilitated microplastic aggregation and deposition in lab experiments, with EPS forming physical bridges between plastic particles and sediment, suggesting that algal biofilm formation can accelerate the settling and burial of buoyant microplastics in aquatic environments.

2021 Chemosphere 89 citations
Article Tier 2

Response of aerobic granular sludge under acute inhibition by polystyrene microplastics: Activity, aggregation performance, and microbial analysis

Researchers investigated how different concentrations of polystyrene microplastics affect the performance of aerobic granular sludge used in wastewater treatment. The study found that increasing microplastic concentrations inhibited nitrogen removal rates and disrupted the microbial community structure within the sludge. Evidence indicates that microplastic contamination in wastewater can compromise the effectiveness of biological treatment processes.

2024 Environmental Pollution 17 citations
Article Tier 2

The effects of microplastics and nanoplastics on nitrogen removal, extracellular polymeric substances and microbial community in sequencing batch reactor

Researchers found that polystyrene nanoplastics and microplastics impaired nitrogen removal in sequencing batch reactors by reducing denitrification rates, altering extracellular polymeric substances, and shifting microbial community composition in activated sludge.

2023 Bioresource Technology 54 citations
Article Tier 2

Exposure to polyamide 66 microplastic leads to effects performance and microbial community structure of aerobic granular sludge

Polyamide 66 microplastics were introduced into aerobic granular sludge bioreactors at varying concentrations, initially reducing contaminant removal efficiency but recovering to near-control levels by the end of the experiment. The study shows that while microplastics transiently disrupt biological wastewater treatment, the microbial community can adapt over time.

2019 Ecotoxicology and Environmental Safety 101 citations
Article Tier 2

Microplastics in granular sequencing batch reactors: Effects on pollutant removal dynamics and the microbial community

Researchers investigated how polyethylene and polyethylene terephthalate microplastics affect pollutant removal in granular sludge wastewater treatment reactors. They found that microplastic type and concentration influenced nitrogen, phosphorus, and organic compound removal rates, with PET particles showing a stronger tendency to accumulate within the biomass. The study indicates that microplastic contamination in wastewater treatment systems may compromise treatment efficiency and alter microbial community dynamics.

2024 Journal of Hazardous Materials 12 citations
Article Tier 2

Impact of phenolic-formaldehyde resin microplastics on anaerobic granular sludge: EPS interaction mechanisms and impacts on reactor performance

Researchers studied how microplastics made from phenolic-formaldehyde resin affect wastewater treatment systems that use anaerobic granular sludge. They found that smaller microplastic particles provided new surfaces for bacteria to grow on, initially boosting treatment performance, but over time the increased microbial activity weakened the structure of the sludge granules. The study reveals a trade-off where microplastics can temporarily improve wastewater processing while ultimately destabilizing the treatment system.

2024 Journal of Hazardous Materials 26 citations
Article Tier 2

[Change in Granulation Potential and Microbial Enrichment Characteristics of Sludge Induced by Microplastics].

This study found that polyethylene terephthalate (PET) microplastics accelerate the formation of granular sludge in wastewater treatment plants by increasing sticky protein secretions, but continuous exposure ultimately degrades treatment performance and disrupts the microbial communities responsible for removing nitrogen. This matters because it suggests microplastics entering sewage systems could compromise the efficiency of the very facilities designed to filter them out.

2023 PubMed
Article Tier 2

Microbial and physicochemical responses of anaerobic hydrogen-producing granular sludge to polyethylene micro(nano)plastics

Researchers found that polyethylene micro- and nanoplastics disrupted anaerobic hydrogen-producing granular sludge in a concentration- and size-dependent manner, inhibiting microbial activity and altering community structure, with nanoplastics causing greater harm than larger microplastic particles.

2022 Water Research 27 citations
Article Tier 2

Effects of microplastics on the properties of different types of sewage sludge and strategies to overcome the inhibition: A review

This review examined how microplastics trapped in sewage sludge during wastewater treatment affect sludge properties, microbial communities, and treatment efficiency, while discussing strategies to overcome microplastic-induced inhibition of sludge processing.

2023 The Science of The Total Environment 44 citations
Article Tier 2

Microplastic isolation method for wastewater and sludge samples by removal of excess organic and inorganic interferences

Researchers developed an improved microplastic isolation method for wastewater and sludge samples that removes extracellular polymeric substances (EPS) interfering with detection. The optimized protocol improves the accuracy and reliability of microplastic quantification in complex environmental matrices.

2023 Chemosphere 8 citations
Article Tier 2

Microplastics reshape frozen algal-bacterial granular reactivation: Decoding structural collapse and microbial drivers of nutrient removal

Researchers investigated how co-existing PET, PVC, and polyethylene microplastics affect the reactivation of frozen algal-bacterial granular sludge used in wastewater treatment, finding that moderate MP concentrations enhanced granular compactness and nitrogen and phosphorus removal while elevated concentrations disrupted structural stability and microbial dynamics. The study identifies MP concentration thresholds critical for maintaining granular sludge function after frozen storage.

2025 Bioresource Technology