We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Papers
61,005 resultsShowing papers similar to Fluorescence radial fluctuation enables two-photon super-resolution microscopy
ClearIn vivo super-resolution of the brain – How to visualize the hidden nanoplasticity?
Researchers reviewed how super-resolution fluorescence microscopy techniques — which allow scientists to image structures smaller than what conventional light microscopes can resolve — are being used to study the nanoscale structure and plasticity of brain synapses in living mice. These imaging advances help reveal how tiny changes in brain connections relate to learning and memory, using "nanoplasticity" in its neurological sense rather than as a reference to plastic pollution.
Imaging dendritic spines in the hippocampus of a living mouse by 3D-STED microscopy
Researchers extended 3D STED super-resolution microscopy to image dendritic spines in the hippocampus of living mice, achieving nanoscale resolution in three dimensions within deep brain tissue and opening new possibilities for studying synaptic structures in vivo.
Cortex-Wide, Cellular-Resolution Volumetric Imaging with a Modular Two-Photon Imaging Platform
Researchers developed Meso2P, a modular two-photon imaging platform that achieves cortex-wide, cellular-resolution volumetric imaging by decoupling excitation and detection using a lateral paraboloid fluorescence collector. The system sustains an effective numerical aperture of 0.87 over a contiguous 6x6 mm2 field-of-view at 7.67 Hz, enabling mapping of neuronal activity across the entire cortex at single-cell resolution.
Cortex-Wide, Cellular-Resolution Volumetric Imaging with a Modular Two-Photon Imaging Platform
This paper presents Meso2P, a new two-photon microscope capable of imaging the entire mouse cortex at single-cell resolution — a significant advance in neuroscience instrumentation. Among its demonstrated applications is the ability to track the distribution of micro- and nanoplastic particles in living brain tissue in real time. While primarily a neuroscience tool, its capacity to visualize nanoplastics in the brain non-invasively could become valuable for directly studying how plastic particles move through and accumulate in neural tissue.
Editorial: 15 years of Frontiers in Cellular Neuroscience: super-resolution microscopy in the healthy and the injured brain
This editorial introduces a research collection on super-resolution microscopy techniques applied to the healthy and injured brain, highlighting how methods that surpass the diffraction limit of classical fluorescence microscopy are revealing new insights into synaptic organization and cellular pathology. The collection covers advances in both imaging hardware and computational image analysis relevant to neuroscience research.
Imaging dendritic spines in the hippocampus of a living mouse by 3D-stimulated emission depletion microscopy
This paper is not about microplastics; it presents an in vivo 3D super-resolution microscopy methodology for imaging dendritic spines in the mouse hippocampus.
Single-Particle Resolution Fluorescence Microscopy of Nanoplastics
Researchers developed a super-resolution fluorescence microscopy technique that enables single-particle detection and precise localization of nanoplastics in biological tissues and environmental samples. This advancement addresses a major limitation in nanoplastic research, as conventional microscopy lacks the resolution to distinguish individual nanoplastics from background fluorescence or free dye.
3D differential interference contrast microscopy using polarisation‐sensitive tomographic diffraction microscopy
Researchers developed a 3D differential interference contrast microscopy technique using tomographic diffraction microscopy to image unlabeled biological and environmental samples at high resolution — with applications for visualizing microplastics in cells and tissues.
Super-resolution imaging of micro- and nanoplastics using confocal Raman with Gaussian surface fitting and deconvolution
Researchers used confocal Raman imaging with Gaussian surface fitting to achieve super-resolution visualization of micro- and nanoplastics beyond the optical diffraction limit, enabling identification and imaging of nanoplastic particles smaller than conventional Raman microscopy can resolve.
Far-field super-resolution chemical microscopy
Researchers reviewed recent advances in "far-field chemical microscopy," a group of techniques that create detailed molecular maps of materials without needing dyes or labels, while also breaking the traditional limits of optical resolution. These super-resolution chemical imaging methods are opening new windows for studying biological systems, identifying environmental contaminants like microplastics, and inspecting materials at the nanoscale.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence, second harmonic generation, and coherent Raman scattering microscopy in a single instrument to image micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics crossed the blood-brain barrier and accumulated in lipid-rich brain regions in mouse models.
Super-resolution Raman imaging towards visualisation of nanoplastics
Super-resolution Raman imaging was evaluated as a method to visualize nanoplastics smaller than the conventional diffraction-limited laser spot size, overcoming a key barrier in nanoplastic characterization. The technique extends confocal Raman capabilities into the nanoscale detection range needed for environmental nanoplastic analysis.
Whole-Tissue Distribution Analysis for Visualization of Nanoplastics in the Mouse Brain
Researchers used whole-tissue clearing combined with fluorescence microscopy to visualize the three-dimensional distribution of nanoplastics throughout intact mouse brains without sectioning. This approach revealed nanoplastic accumulation patterns across brain regions that section-based imaging would have missed, demonstrating a valuable method for mapping nanoplastic biodistribution in structurally complex organs.
Raman spectroscopy as the quantum eye to reveal molecular dynamics in biology
Researchers reviewed advances in Raman spectroscopy — a technique that identifies chemicals by how they scatter laser light — highlighting how recent innovations in surface-enhanced and stimulated Raman methods have expanded its applications in cell imaging, disease diagnosis, drug development, and microplastic detection.
Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging
Researchers developed Signal Improved ultra-Fast Light-sheet Microscope (SIFT) using two fixed distant light-sheet foci to enable axially swept light-sheet microscopy (ASLM) at 40 frames per second across a full field of view, achieving four-fold speed improvement over current state-of-the-art for large cleared tissue imaging.
Signal improved ultra-fast light-sheet microscope for large tissue imaging
Researchers developed an improved light-sheet microscope that images large tissue samples four times faster than current methods while also doubling the signal strength, using a deep learning algorithm to better identify tissue boundaries — advancing the ability to create detailed 3D maps of biological tissues.
Synchrotron-based Spectromicroscopy for Microplastic Detection and Characterization
Researchers reviewed how synchrotron-based imaging techniques — which use powerful X-ray beams to see extremely fine details — can detect and chemically identify micro- and nanoplastics that conventional methods miss, including plastics absorbed into biological tissues. These high-resolution tools are still in early stages but show strong potential for mapping microplastic contamination at the nanoscale.
From Local to Global: Efficient Dual Attention Mechanism for Single Image Super-Resolution
Researchers developed a dual attention mechanism for deep learning neural networks to improve single image super-resolution. This type of image enhancement technology could have applications in improving the detection and classification of microplastic particles in environmental images.
White matter hyperintensities and microplastics
Researchers aligned ante-mortem and post-mortem brain MRI scans and found large amounts of plastic particles in brain regions showing white matter hyperintensities, which are associated with small vessel disease. Using a novel optical imaging approach, they identified the cellular locations of these plastics in areas with vascular injury and amyloid plaques. The study raises important questions about whether microplastics in the brain contribute to or result from pre-existing vascular damage in people with cognitive impairment.
Broadband background-free stimulated Raman scattering microspectroscopy with a novel frequency modulation scheme
Researchers developed broadband background-free stimulated Raman scattering microspectroscopy using a novel laser system, enabling chemical imaging without the fluorescence background that limits conventional Raman measurements. The technique offers improved sensitivity for detecting microplastics and other materials in complex biological samples.
Uncovering Hidden Dynamics of Natural Photonic Structures Using Holographic Imaging
Researchers used holography and optics to reveal hidden nanoscale dynamics in natural photonic structures — biological surfaces that manipulate light through intricate nano-architectures. These optical techniques could be adapted for characterizing the fine structure of microplastic particles.
Signal Improved ultra-Fast Light-sheet Microscope (SIFT) for large tissue imaging
Researchers developed an improved light-sheet microscopy system that can image large tissue samples faster while maintaining high resolution. Advanced imaging tools like this are being adapted to detect and characterize microplastic particles lodged in biological tissues, which is important for assessing health impacts.
Correlative spectroscopy and microscopy analysis of micro- and nanoplastics in complex biological matrices
Researchers combined fluorescence microscopy, second harmonic generation imaging, and coherent Raman scattering to detect and map micro- and nanoplastics in lung cells, zebrafish, and mouse tissues. Polystyrene nanoplastics were found to cross the blood-brain barrier and accumulate in lipid-rich brain regions in animal models.
On the use of deep learning for phase recovery
Researchers reviewed how deep learning — a type of artificial intelligence — can recover phase information from light, which is typically lost when cameras capture images, enabling sharper microscopy and better materials analysis. These advances improve the tools scientists use to study tiny particles, including microplastics, at very fine scales.