Papers

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Article Tier 2

Response of aerobic granular sludge under polyethylene microplastics stress: Physicochemical properties, decontamination performance, and microbial community

Researchers investigated the impact of polyethylene microplastics on aerobic granular sludge used in wastewater treatment. The study found that microplastics significantly disrupted sludge structure, settling properties, and enzyme activities related to denitrification and phosphorus removal, with increased reactive oxygen species and cell membrane damage at higher concentrations.

2022 Journal of Environmental Management 79 citations
Article Tier 2

Revealing the influencing mechanisms of polystyrene microplastics (MPs) on the performance and stability of the algal-bacterial granular sludge

Researchers investigated how polystyrene microplastics affect algal-bacterial granular sludge used in wastewater treatment, finding that the sludge removed over 96% of incoming microplastics but that microplastics inhibited COD removal by 2.6 to 4.1% and total phosphorus removal by 2.9 to 5.8%. Structural stability was compromised through oxidative stress, altered protein composition, and reduced abundance of key functional bacteria.

2022 Bioresource Technology 73 citations
Article Tier 2

Adaptation responses of microalgal-bacterial granular sludge to polystyrene microplastic particles in municipal wastewater

Researchers found that polystyrene microplastics of varying sizes did not significantly impair organic, ammonia, or phosphorus removal in microalgal-bacterial granular sludge systems treating municipal wastewater, though the microplastics did alter microbial community composition.

2022 Environmental Science and Pollution Research 28 citations
Article Tier 2

Mechanisms underlying the detrimental impact of micro(nano)plastics on the stability of aerobic granular sludge: Interactions between micro(nano)plastics and extracellular polymeric substances

Researchers found that both micro- and nanoplastics at realistic concentrations harmed the performance of aerobic granular sludge, a technology used for wastewater treatment, by reducing its ability to remove nitrogen. The plastic particles interacted with the sticky substances that hold the sludge granules together, weakening their structural integrity. The study reveals a specific mechanism by which plastic pollution can undermine wastewater treatment systems that communities rely on for clean water.

2024 Journal of Hazardous Materials 31 citations
Article Tier 2

The effects of microplastics and nanoplastics on nitrogen removal, extracellular polymeric substances and microbial community in sequencing batch reactor

Researchers found that polystyrene nanoplastics and microplastics impaired nitrogen removal in sequencing batch reactors by reducing denitrification rates, altering extracellular polymeric substances, and shifting microbial community composition in activated sludge.

2023 Bioresource Technology 54 citations
Article Tier 2

Polystyrene nanoplastics reshape the anaerobic granular sludge for recovering methane from wastewater

Researchers investigated the long-term effects of polystyrene nanoplastics on anaerobic granular sludge used for methane recovery from wastewater over a 120-day continuous test. The study found that while low nanoplastic concentrations had minimal impact, higher concentrations reshaped the microbial community structure and altered sludge performance, raising concerns about nanoplastic effects on wastewater treatment processes.

2020 Water Research 170 citations
Article Tier 2

Different sizes of polystyrene microplastics induced distinct microbial responses of anaerobic granular sludge

Researchers exposed anaerobic granular sludge used in wastewater treatment to polystyrene microplastics of different sizes, ranging from 0.5 to 150 micrometers. They found that larger particles caused progressively greater inhibition of methane production, with distinct microbial community shifts depending on particle size. The study reveals that microplastic size is an important factor in determining the severity of disruption to anaerobic wastewater treatment processes.

2022 Water Research 73 citations
Article Tier 2

The impacts of biodegradable and non-biodegradable microplastic on the performance and microbial community characterization of aerobic granular sludge

Researchers compared the effects of biodegradable polylactic acid microplastics and non-biodegradable polyethylene microplastics on aerobic granular sludge used in wastewater treatment. They found that high concentrations of both types impaired the sludge's ability to remove organic pollutants, but both actually enhanced nitrogen and phosphorus removal at moderate levels. The study reveals that even biodegradable microplastics can disrupt wastewater treatment processes in unexpected ways.

2024 Frontiers in Microbiology 14 citations
Article Tier 2

Exposure to nanoplastic induces cell damage and nitrogen inhibition of activated sludge: Evidence from bacterial individuals and groups

Researchers exposed activated sludge in a wastewater treatment reactor to polystyrene nanoplastics at concentrations up to 10 mg/L over 30 days. They found that nanoplastic exposure caused cell membrane damage, increased oxidative stress, and significantly inhibited nitrogen removal processes. The study suggests that nanoplastic accumulation in wastewater treatment plants could compromise their ability to effectively process nitrogen-containing pollutants.

2022 Environmental Pollution 52 citations
Article Tier 2

Synergistic analysis of performance, microbial community, and metabolism in aerobic granular sludge under polyacrylonitrile microplastics stress

Researchers found that low concentrations of polyacrylonitrile microplastics had minimal impact on aerobic granular sludge wastewater treatment, but 100 mg/L severely damaged granule structure and inhibited denitrification and membrane transport gene expression.

2023 Bioresource Technology 30 citations
Article Tier 2

Deciphering the role of polystyrene microplastics in waste activated sludge anaerobic digestion: Changes of organics transformation, microbial community and metabolic pathway

Researchers found that polystyrene microplastics in sewage sludge affected the anaerobic digestion process used to treat waste, with low concentrations slightly boosting methane production but high concentrations reducing it by up to 11%. The microplastics disrupted key bacterial communities and enzyme activities needed for proper waste breakdown. This matters because wastewater treatment plants handle enormous volumes of microplastic-laden sludge, and impaired digestion could reduce treatment effectiveness and release more pollutants into the environment.

2023 The Science of The Total Environment 43 citations
Article Tier 2

Effect evaluation of microplastics on activated sludge nitrification and denitrification

Researchers found that microplastics entering wastewater treatment plants interfere with the nitrification and denitrification processes carried out by activated sludge microbes, potentially reducing the effectiveness of nutrient removal in sewage treatment. This effect could undermine water quality if microplastic loads in wastewater continue to increase.

2019 The Science of The Total Environment 172 citations
Article Tier 2

Impact of Polyethylene Terephthalate Microplastics on Aerobic Granular Sludge Structure and EPS Composition in Wastewater Treatment

Researchers investigated how PET microplastics affect the structure and function of aerobic granular sludge used in wastewater treatment. Higher microplastic concentrations led to changes in granule size, altered the composition of extracellular polymeric substances, and shifted microbial community structure. The findings suggest that microplastic contamination in wastewater could compromise the stability and efficiency of biological treatment processes.

2025 Water 6 citations
Article Tier 2

Impact of the concentration and type of microplastics on the treatment efficiency and biomass structure in aerobic granular sludge reactors

Researchers investigated how polyethylene terephthalate and polyethylene microplastics at varying concentrations affect treatment efficiency, granule morphology, extracellular polymer production, microbial species composition, and metabolic activity in aerobic granular sludge reactors used for wastewater treatment. The study tested four hypotheses regarding MP effects on both the biological performance and structural integrity of aerobic granular sludge as a promising technology for MP-contaminated wastewater.

2022 Zenodo (CERN European Organization for Nuclear Research)
Article Tier 2

Micro- and nanoplastics in granular sludge systems: mechanisms of disruption, retention, and microbial adaptation in wastewater treatment technologies

This review examines how micro- and nanoplastics disrupt the biological systems used to treat wastewater, focusing on granular sludge technologies. Plastic particles damage the microbial communities that break down waste by causing oxidative stress and breaking apart the protective structures that hold bacteria together. This matters because if wastewater treatment becomes less effective due to plastic contamination, more pollutants including microplastics could pass through into waterways that supply drinking water.

2025 Water Research X 5 citations
Article Tier 2

Effect of microplastic particle size to the nutrients removal in activated sludge system

This study investigated how different polystyrene microplastic particle sizes affect nitrogen and phosphorus removal in activated sludge systems. Results showed that microplastic size influenced ammonia oxidation, nitrite and nitrate cycling, and phosphorus removal, with finer particles causing greater disruption to biological nutrient removal.

2021 Marine Pollution Bulletin 50 citations
Article Tier 2

Microplastics in granular sequencing batch reactors: Effects on pollutant removal dynamics and the microbial community

Researchers investigated how polyethylene and polyethylene terephthalate microplastics affect pollutant removal in granular sludge wastewater treatment reactors. They found that microplastic type and concentration influenced nitrogen, phosphorus, and organic compound removal rates, with PET particles showing a stronger tendency to accumulate within the biomass. The study indicates that microplastic contamination in wastewater treatment systems may compromise treatment efficiency and alter microbial community dynamics.

2024 Journal of Hazardous Materials 12 citations
Article Tier 2

Exposure to polyamide 66 microplastic leads to effects performance and microbial community structure of aerobic granular sludge

Polyamide 66 microplastics were introduced into aerobic granular sludge bioreactors at varying concentrations, initially reducing contaminant removal efficiency but recovering to near-control levels by the end of the experiment. The study shows that while microplastics transiently disrupt biological wastewater treatment, the microbial community can adapt over time.

2019 Ecotoxicology and Environmental Safety 101 citations
Article Tier 2

Metagenomic analysis reveals the responses of microbial communities and nitrogen metabolic pathways to polystyrene micro(nano)plastics in activated sludge systems

Scientists used genetic analysis to study how polystyrene micro- and nanoplastics disrupt the bacteria that process nitrogen in wastewater treatment systems. At high concentrations, the plastics reduced nitrogen removal efficiency by up to 30% by generating harmful reactive oxygen species that damaged key microbial processes. This is concerning because less effective wastewater treatment means more pollutants, including microplastics themselves, could end up in waterways.

2023 Water Research 135 citations
Article Tier 2

Effects of degradable and non-degradable microplastics on SPNEDPR-AGS system: Sludge characteristics, nutrient transformation, key enzyme, and microbial community

Researchers examined how degradable and non-degradable microplastics affect an aerobic granular sludge (AGS) wastewater treatment system, measuring impacts on granule formation, microbial community, and treatment performance. Degradable microplastics caused more pronounced disruption to the AGS system than conventional non-degradable plastics.

2024 Bioresource Technology 9 citations
Article Tier 2

Microplastics shaped performance, microbial ecology and community assembly in simultaneous nitrification, denitrification and phosphorus removal process

This study found that polystyrene and PVC microplastics disrupted the performance of wastewater treatment systems designed to remove nitrogen and phosphorus, reducing nitrogen removal by up to 10%. The microplastics altered microbial communities, decreased cooperation between beneficial bacteria, and blocked important biological pathways. Since wastewater treatment is a key barrier against pollution reaching drinking water, microplastic interference with these systems could indirectly increase human exposure to harmful contaminants.

2024 The Science of The Total Environment 12 citations
Article Tier 2

Insight into effect of polyethylene microplastic on nitrogen removal in moving bed biofilm reactor: Focusing on microbial community and species interactions

Researchers studied how polyethylene microplastics affect nitrogen removal in wastewater treatment bioreactors and found that low concentrations slightly improved the process, while higher concentrations disrupted it. The microplastics changed the microbial communities responsible for breaking down nitrogen in wastewater. This matters because less effective wastewater treatment means more nitrogen pollution in waterways, and microplastics entering treatment plants could reduce their ability to clean water effectively.

2024 The Science of The Total Environment 17 citations
Article Tier 2

Effects of exposure to polyether sulfone microplastic on the nitrifying process and microbial community structure in aerobic granular sludge

Scientists added polyether sulfone microplastics to aerobic granular sludge bioreactors at different concentrations and found only minor effects on ammonia removal but an increase in total nitrogen removal efficiency of 5.6%, along with shifts in nitrifying microbial community structure.

2020 Bioresource Technology 110 citations
Article Tier 2

Influence of polystyrene microplastics on the structural stability of activated sludge microbial flocs in sequencing batch reactors

Researchers exposed sequencing batch reactors to polystyrene microplastics at 0, 10, and 100 mg/L and measured effects on activated sludge floc structure. Microplastic exposure caused a 7.8-13.3% reduction in floc density and a significant rise in effluent turbidity, impairing sludge settling and potentially reducing wastewater treatment efficiency.

2025 Environmental Pollution