Papers

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Article Tier 2

Longitudinal patterns of microplastic concentration and bacterial assemblages in surface and benthic habitats of an urban river

This study measured microplastic concentrations and microbial communities in a river from source to mouth, finding that both plastic levels and unique plastisphere bacterial communities increased downstream of wastewater treatment plant outflows. The results identify wastewater discharge as a key driver of both microplastic loading and microbial community shifts in rivers.

2017 Freshwater Science 184 citations
Article Tier 2

Metagenomic insights into environmental risk of field microplastics in an urban river

Metagenomic analysis of microplastics sampled along an urban river watershed revealed that MP-associated microbial communities carried antibiotic resistance genes and virulence factors at higher levels than surrounding water, with composition shifting along the river gradient. The findings confirm microplastics as environmental vectors for spreading antimicrobial resistance.

2022 Water Research 63 citations
Article Tier 2

Antibiotic resistance genes and virulence factors in the plastisphere in wastewater treatment plant effluent: Health risk quantification and driving mechanism interpretation

Researchers found that microplastics in treated wastewater carry significantly more disease-causing bacteria, antibiotic resistance genes, and virulence factors on their surfaces compared to the surrounding water. This means microplastics released from wastewater treatment plants into rivers and lakes could spread antibiotic-resistant infections, posing a direct risk to communities that rely on these water sources.

2024 Water Research 54 citations
Article Tier 2

Wastewater discharges and polymer type modulate the riverine plastisphere and set the role of microplastics as vectors of pathogens and antibiotic resistance

Researchers investigated how wastewater treatment plant discharges and polymer type shape microbial communities on microplastics in a river environment. They found that microplastics harbored significantly higher microbial diversity than surrounding water, and that wastewater discharges led to a 2.3-fold increase in antibiotic resistance gene abundance on the plastic surfaces. Different polymer types, including polyethylene, polypropylene, and PET, each attracted distinct microbial communities with varying levels of pathogens and resistance genes.

2025 Journal of Water Process Engineering 1 citations
Article Tier 2

Increased inheritance of structure and function of bacterial communities and pathogen propagation in plastisphere along a river with increasing antibiotics pollution gradient.

This study examined how bacterial communities colonizing plastic debris in a river — the Plastisphere — change along a gradient of increasing antibiotic pollution. Plastic debris hosted distinct microbial communities compared to surrounding water, and areas with higher antibiotic levels showed greater inheritance of resistant bacterial structures on plastic surfaces, suggesting plastics facilitate the spread of antibiotic resistance.

2020 Environmental pollution (Barking, Essex : 1987)
Article Tier 2

Metagenomic insights into ecological risk of antibiotic resistome and mobilome in riverine plastisphere under impact of urbanization

This study used advanced genetic sequencing to examine antibiotic resistance genes on microplastics found in an urban river. Microplastics harbored more antibiotic resistance genes and mobile genetic elements than natural materials like rocks and wood, and the problem was worse in more urbanized areas. The findings suggest that microplastics in waterways can act as hotspots for spreading antibiotic resistance, which is a growing public health threat.

2024 Environment International 11 citations
Article Tier 2

Potential routes of plastics biotransformation involving novel plastizymes revealed by global multi-omic analysis of plastic associated microbes

Researchers analyzed all publicly available genetic data from microbes living on plastic debris worldwide and found that while plastic-eating bacteria are rare in most environments, rivers appear to be hotspots for novel plastic-degrading organisms. They also created a freely accessible database of these plastic-associated microbes, which could accelerate efforts to develop biological solutions for plastic pollution.

2024 Scientific Reports 15 citations
Article Tier 2

Plastisphere showing unique microbiome and resistome different from activated sludge

Researchers used metagenomics to compare the microbiome and resistome of PVC plastisphere biofilms with activated sludge, finding that microplastic surfaces enriched distinct pathogenic bacteria and antibiotic resistance genes that differ from the surrounding sludge community.

2022 The Science of The Total Environment 32 citations
Article Tier 2

Impact of Urbanization on Antibiotic Resistome in Different Microplastics: Evidence from a Large-Scale Whole River Analysis

Researchers conducted a large-scale river survey across urbanization gradients and characterized antibiotic resistance genes on microplastics from each zone, finding that urbanization level strongly predicted the diversity and abundance of resistance genes on plastic surfaces.

2021 Environmental Science & Technology 100 citations
Article Tier 2

Metagenomics reveals combined effects of microplastics and antibiotics on microbial community structure and function in coastal sediments

A metagenomic study of coastal sediments exposed to combined microplastic and antibiotic pollution found that co-exposure altered microbial community composition and significantly elevated the abundance and diversity of antibiotic resistance genes compared to either pollutant alone.

2025 Marine Pollution Bulletin 3 citations
Article Tier 2

Unraveling the role of microplastics in antibiotic resistance: Insights from long-read metagenomics on ARG mobility and host dynamics

Researchers used long-read metagenomics to investigate how microplastics serve as vectors for antibiotic resistance genes in aquatic environments. They found that plasmid-encoded resistance genes varied significantly between microplastic biofilms and surrounding water, highlighting horizontal gene transfer as a key mechanism for resistance gene enrichment on plastic surfaces. The study identified specific bacterial taxa driving this enrichment and revealed that enhanced cell adhesion and transporter activity on microplastics facilitate the spread of antibiotic resistance.

2025 Journal of Hazardous Materials 5 citations
Article Tier 2

Impact of sulfamethoxazole on a riverine microbiome

Scientists studied how the antibiotic sulfamethoxazole affects the microbial community in a river, finding that even low concentrations shifted the balance of bacteria and promoted antibiotic resistance genes. This is relevant to the microplastic field because both antibiotics and microplastics promote antibiotic resistance when they co-occur in aquatic environments.

2021 1 citations
Article Tier 2

Untargeted metabolomic insights into plastisphere communities in European rivers

Researchers used untargeted metabolomics to characterize plastisphere microbial communities colonizing polyethylene-based plastic pellets in European rivers, simulating microplastic transport between freshwater and marine ecosystems to understand how the plastisphere microbiome and its metabolic outputs shift across environmental transitions.

2024 Environmental Science and Pollution Research 1 citations
Article Tier 2

Evaluating the role of microplastics and wastewater in shaping Vibrio spp. and antibiotic resistance gene abundance in urban freshwaters

Researchers sampled water and microplastic biofilms from urban South African rivers and found that microplastics disproportionately enriched Vibrio spp. and tetracycline resistance genes relative to the surrounding water, suggesting microplastics selectively concentrate pathogens and antibiotic resistance genes.

2025 Scientific Reports
Article Tier 2

Distinct profile of bacterial community and antibiotic resistance genes on microplastics in Ganjiang River at the watershed level

Researchers investigated microplastic pollution and associated bacterial communities, human pathogenic bacteria, and antibiotic resistance genes across the Ganjiang River watershed. They found microplastics were widely distributed with an average of 407 particles per cubic meter, and that microplastic surfaces harbored significantly higher bacterial diversity and more antibiotic resistance genes than surrounding water or sediment.

2021 Environmental Research 92 citations
Article Tier 2

Evidence of selective enrichment of bacterial assemblages and antibiotic resistant genes by microplastics in urban rivers

Researchers sampled microplastics from two urban rivers in China and found that the bacterial communities colonizing plastic particles were distinctly different from those in the surrounding water. The microplastic-associated bacteria had lower diversity but higher proportions of biofilm-forming species and functions linked to human disease. Notably, the study found that microplastics selectively enriched antibiotic resistance genes, raising concerns about plastics serving as reservoirs for drug-resistant bacteria.

2020 Water Research 313 citations
Article Tier 2

Deciphering the mechanisms shaping the plastisphere antibiotic resistome on riverine microplastics

Researchers found that microplastics in China's Huangpu River selectively enrich antibiotic resistance genes for Rifamycin and Vancomycin, creating unique bacterial niches that favor horizontal gene transfer and dissemination of resistance through stochastic assembly processes.

2022 Water Research 87 citations
Article Tier 2

Deciphering the distinct successional patterns and potential roles of abundant and rare microbial taxa of urban riverine plastisphere

Researchers examined how microbial communities colonize microplastics in urban river environments, distinguishing between abundant and rare bacterial species. The study found that rare taxa played critical roles in maintaining community stability on plastic surfaces, while abundant taxa drove community succession, and both groups contributed to nutrient cycling functions.

2023 Journal of Hazardous Materials 69 citations
Article Tier 2

Ecological Rolesand Shared Microbes Differentiatethe Plastisphere from Natural Particle-Associated Microbiomes in UrbanRivers

Researchers compared the microbiomes on microplastics (the 'plastisphere') versus natural particles in ten urban rivers using metagenomics, finding similar overall taxonomic and functional compositions between the two. However, the plastisphere harbored distinct specialist taxa with enhanced capacity for complex carbohydrate metabolism and unique ecological strategies.

2025 Figshare
Article Tier 2

Disentangling abiotic and biotic effects of treated wastewater on stream biofilm resistomes enables the discovery of a new planctomycete beta-lactamase

This paper is not about microplastics — it investigates how wastewater discharge shapes antibiotic resistance gene (ARG) communities in stream biofilms, finding that biological (microbial community) factors dominate over chemical factors, and reports the discovery of a novel functional beta-lactamase enzyme from a rarely studied bacterial phylum.

2023 2 citations
Article Tier 2

Identification of microplastic-associated microbial communities from various stages of wastewater treatment and recipient surface waters using MALDI-TOF mass spectrometry

Researchers deployed six polymer types at different stages of wastewater treatment across three Hungarian plants and used MALDI-TOF mass spectrometry to identify the bacteria colonizing microplastic surfaces, finding distinct microbial communities that may act as vectors for antibiotic resistance.

2025 Journal of Central European Green Innovation
Article Tier 2

Contribution of microplastic particles to the spread of resistances and pathogenic bacteria in treated wastewaters

Researchers studied microplastic particles collected from treated wastewater effluents and found that MPs harbored significantly higher loads of antibiotic resistance genes and pathogenic bacteria compared to surrounding water, suggesting MPs facilitate their environmental spread.

2021 Water Research 140 citations
Article Tier 2

Microplastics accumulate priority antibiotic-resistant pathogens: Evidence from the riverine plastisphere

Researchers placed microplastics in river water and found they accumulated more antibiotic-resistant bacteria than natural sand particles, including dangerous pathogens like E. coli and Klebsiella. Most of the bacteria isolated from the plastic surfaces were multi-drug resistant and carried virulence traits like biofilm formation. This suggests microplastics in waterways may act as rafts for spreading antibiotic resistance through the environment.

2023 Environmental Pollution 58 citations
Article Tier 2

Microplastic is an Abundant and Distinct Microbial Habitat in an Urban River

Researchers demonstrated that microplastic surfaces in an urban river host a microbial community that is distinct from surrounding water and sediment communities, establishing microplastic as an abundant and ecologically distinct habitat for river microorganisms.

2014 Environmental Science & Technology 1428 citations