We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
ProteinMicroplasticCoronation Complexes TriggerProteome Changes in Brain-Derived Neuronal and Glial Cells
Summary
Researchers used mass spectrometry-based proteomics to compare how intact polystyrene microplastics versus protein-coated (coronated) microplastics affect brain-derived neuronal and glial cells. Protein corona formation on microplastics notably altered cellular protein expression compared to uncoated particles, with impacts on DNA repair, oxidative stress response, and membrane trafficking pathways.
The extensive distribution of microplastics (MPs) in the environment and their food chain contamination urgently necessitates a deeper understanding of their molecular-level impact on physiological responses. This study employed a mass spectrometry-based proteomics approach to investigate the potential risks, mechanisms of associated cellular processes, and biological reactions to preformed protein-MPs coronation and intact MPs using brain-derived neuronal and glial cells. Our findings indicate that MPs can adsorb proteins and form a heterogeneous corona layer when interacting with biological fluids such as serum. Proteomics analysis revealed that protein–MP coronation notably alters protein expression levels compared to intact MPs, impacting core cellular biological processes, including protein synthesis machinery and RNA processing pathways, lipid metabolism, and nuclear–cytoplasmic compartmentalization and transport. Notably, the heterogeneous protein adsorption onto MP surfaces perturbs a wide range of cellular signaling pathways through cellular recognition mechanisms, potentially contributing to the challenge of MP accumulation in the brain.
Sign in to start a discussion.
More Papers Like This
Protein Microplastic Coronation Complexes Trigger Proteome Changes in Brain-Derived Neuronal and Glial Cells
Researchers used a proteomics approach to study how microplastics interact with brain-derived neuronal and glial cells, finding that the particles adsorb proteins from biological fluids to form a coating called a protein corona. This corona significantly altered protein expression in cells compared to bare microplastic particles, affecting protein synthesis, lipid metabolism, and cellular transport processes. The study suggests that the protein corona on microplastics may play a key role in how these particles affect brain cells.
ProteinMicroplasticCoronation Complexes TriggerProteome Changes in Brain-Derived Neuronal and Glial Cells
This is a duplicate entry for the protein-microplastic corona proteomics study (same paper as ID 9703), investigating how protein coronation of polystyrene microplastics triggers distinct proteome changes in neuronal and glial cells compared to bare microplastics.
Fate of polystyrene micro- and nanoplastics in zebrafish liver cells: Influence of protein corona on transport, oxidative stress, and glycolipid metabolism
Scientists studied how proteins in biological fluids coat nanoplastic particles (forming a "protein corona") and how this coating changes the way cells take up and process the plastics. The protein coating actually increased how many nanoplastics entered liver cells and made them harder to clear out, suggesting that once nanoplastics enter the bloodstream, the body's own proteins may make the contamination harder to eliminate.
Unravelling protein corona formation on pristine and leached microplastics
Researchers found that when microplastics encounter proteins in biological fluids, they get coated in a "protein corona" that depends heavily on the plastic's chemical additives, surface area, and how much it has been weathered in the environment. This coating changes how microplastics behave in the body, meaning toxicity studies need to account for these real-world surface changes.
Unravelling protein corona formation on pristine and leached microplastics
When microplastics enter biological fluids or protein-rich environments, proteins coat their surface to form a 'protein corona' that changes how the particles behave in living systems. This study explored how the physical and chemical properties of pristine versus weathered microplastics influence corona formation, finding that surface changes caused by environmental aging significantly alter protein binding. Understanding this process matters because the protein coat — not the plastic itself — is often what cells and organisms first encounter.