Connecting agrobacterium tumefaciens for bio-degradation of low-density polyethylene: opportunities and challenges ahead

LDPE contributes significantly to global plastic waste accumulation, necessitating effective biodegradation strategies.Its persistence in ecosystems raises concerns, prompting the need for innovative solutions.Microbial degradation of environmental pollutants is a widely accepted phenomenon, driven by the decomposer nature of naturally occurring microorganisms.This study aimed to explore Agrobacterium tumefaciens for bio-degradation of low-density polyethylene (LDPE).Indigenous bacteria capable of LDPE degradation were isolated from a solid waste dumping site in Durg district and screened for their biodegradation potential using PEG assays.The research highlights that the study site sheltered a well-known genetic engineer Agrobacterium tumefaciens capable of degrading LDPE effectively.Molecular characterization supported the identification of potential isolate as Agrobacterium tumefaciens, providing authentic nomenclature for the microbial agent involved in LDPE degradation.The degradation process was monitored through various methods, including weight loss measurements and pH changes at regular intervals, which confirmed the ongoing bio-degradation of LDPE.Furthermore, Fourier Transform Infrared Spectroscopy (FTIR) analyses revealed chemical changes in the pollutant, leading to the formation of simpler compounds that could potentially be utilized or removed by other environmental organisms.Additionally, Scanning Electron Microscopy (SEM) studies provided crucial visual evidence of structural alterations on the LDPE surface due to microbial activity.SEM images depicted the formation of pits, cracks, and other signs of surface degradation, illustrating the physical changes occurring during the biodegradation process.These findings emphasize the significant potential of indigenous bacterial strains in contributing to the remediation of LDPE pollution through microbial degradation processes.