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Single and combined effects of amino polystyrene and perfluorooctane sulfonate on hydrogen-producing thermophilic bacteria and the interaction mechanisms.

The Science of the total environment 2020 Score: 30 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Wanying Chen, Dong Yuan, Min Shan, Zhongbao Yang, Chunguang Liu

Summary

This study examined how amino polystyrene nanoplastics and the industrial chemical PFOS interact with hydrogen-producing bacteria used in bioenergy production, finding that nanoplastics disrupted bacterial metabolism and their combined toxicity with PFOS was greater than either alone. The results raise concerns about the impact of nanoplastic contamination on anaerobic bioprocesses used for renewable energy.

Polymers

As a carrier of perfluorooctane sulfonate, nano-plastics are ubiquitous and finally enriched in the sludge, which is widely used as a raw material for the production of bioenergy (hydrogen or methane) by anaerobic digestion. However, there are still many unknowns about their metabolic toxicity to functional microbes (e.g. hydrogen-producing thermophilic bacteria). Therefore, single and combined effects of amino polystyrene (NPS: 70 nm; 0.2 mg/L) and perfluorooctane sulfonate (PFOS: 0.1, 1 and 5 mg/L) on hydrogen-producing thermophilic bacteria were investigated after exposure for 7 days at 55 °C and pH = 5.7. Single NPS exhibited obvious interference to the metabolism of thermophilic bacteria, resulting in a 53.9% reduction in hydrogen production. However, the combined NPS + PFOS produced an antagonistic effect, leading to a 31.6% reduction in hydrogen production. Nonetheless, the single and combined exposure did not alter the type of hydrogen production (acetic acid-type hydrogen fermentation). Moreover, single NPS and combined NPS + PFOS not only induced the changes of the composition of extracellular polymers (EPSs) and π bond structure of the protein in EPSs, but also decreased the activity of hydrolase in EPSs and surface charge of EPSs. Compared to single NPS exposure, NPS + PFOS-exposed thermophilic bacteria was less permeable to a semi-membrane permeable dye and produced less reactive oxygen species, but were still significantly higher than control group. In short, the main mechanisms of single NPS and combined NPS + PFOS were both to increase cell permeability and to induce oxidative stress. The addition of PFOS alleviated the toxic effect of NPS, but did not change its mechanism of toxicity.

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