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Automated Image Analysis of CNS Cell Cultures Exposed to Microplastics
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Researchers exposed primary cultures of central nervous system neurons and glial cells to polyethylene terephthalate and polypropylene micro- and nanoplastics at 100 and 500 μg/mL concentrations for 28 days, using phase-contrast microscopy and automated FIJI image analysis to quantify morphological changes. They found that PET at 100 μg/mL and polypropylene at 500 μg/mL produced the most significant morphological alterations, including increased cell-free zones and glial proliferation indicative of cellular stress and glial reactivity.
Este estudio evalúa los efectos de micro- y nanoplásticos de tereftalato de polietileno (PET) y polipropileno (PP) sobre cultivos primarios de células del sistema nervioso central (SNC), compuestos por neuronas y células gliales. Las células fueron expuestas durante 28 días a concentraciones de 100 y 500 µg/mL de PET y PP. Las imágenes obtenidas mediante microscopía de contraste de fases se analizaron con FIJI para cuantificar áreas vacías y la proliferación glial. Los resultados mostraron que PET100 y PP500 provocaron las alteraciones morfológicas más significativas, con aumento de zonas sin células y de células gliales, lo que sugiere una respuesta de estrés celular asociada a reactividad glial. La metodología aplicada permitió un seguimiento no invasivo a lo largo del tiempo, sin afectar la viabilidad celular. Estos hallazgos subrayan el potencial impacto neurotóxico de los microplásticos y respaldan el uso del análisis automatizado de imágenes como herramienta útil para evaluar contaminantes ambientales en modelos in vitro del SNC.
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