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Flow cytometry combined with viSNE for the analysis of microbial biofilms and detection of microplastics
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Researchers developed a stain-free flow cytometry method combined with viSNE dimensionality reduction for analyzing stream biofilms at the individual cell level, demonstrating its ability to track community structure, phototrophic decay, and microplastic contamination in both laboratory experiments and field samples.
Biofilms serve essential ecosystem functions and are used in different technical applications. Studies from stream ecology and waste-water treatment have shown that biofilm functionality depends to a great extent on community structure. Here we present a fast and easy-to-use method for individual cell-based analysis of stream biofilms, based on stain-free flow cytometry and visualization of the high-dimensional data by viSNE. The method allows the combined assessment of community structure, decay of phototrophic organisms and presence of abiotic particles. In laboratory experiments, it allows quantification of cellular decay and detection of survival of larger cells after temperature stress, while in the field it enables detection of community structure changes that correlate with known environmental drivers (flow conditions, dissolved organic carbon, calcium) and detection of microplastic contamination. The method can potentially be applied to other biofilm types, for example, for inferring community structure for environmental and industrial research and monitoring.
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Flow cytometry as new promising detection tool for micro and submicron plastic particles
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Researchers evaluated flow cytometry as a tool for detecting and counting micro- and submicron plastic particles in environmental and biological samples. The method offered rapid throughput and the ability to distinguish plastic particles from biological material, but required careful optimization for complex matrices.
Multidimensional analysis methods for flow cytometry : Pushing the boundaries
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This thesis developed new methods for analyzing multidimensional flow cytometry data to better identify cell populations. While a bioinformatics and immunology paper, flow cytometry is also used in cutting-edge research to detect and quantify micro- and nanoplastics in biological fluids.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
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Researchers evaluated flow cytometry as a detection tool for micro- and nanoplastics, testing its ability to rapidly identify and count plastic particles in environmental and biological samples. Results demonstrated that flow cytometry offers a promising high-throughput approach for microplastic detection compared to more time-intensive conventional methods.
Can flow cytometry emerge as a high-throughput technique for micro- and nanoplastics analysis in complex environmental aqueous matrices?
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Researchers reviewed the potential of flow cytometry — a technique that rapidly analyzes individual particles — as a high-throughput tool for detecting micro- and nanoplastics in water samples, finding it excels at measuring particles smaller than 20 micrometers that other methods struggle to detect. Using fluorescent dyes to tag plastics, the approach could enable near-real-time environmental monitoring at a scale no other current technique can match.
Role of Flow Cytometry in the Analysis of Micro/Nano Plastics
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Researchers reviewed how flow cytometry — a technique that uses light beams to rapidly analyze individual particles — can be adapted to identify and count microplastic and nanoplastic particles, potentially offering a faster and more automated approach to pollution monitoring.
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When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.