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Role of Flow Cytometry in the Analysis of Micro/Nano Plastics
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Researchers reviewed how flow cytometry — a technique that uses light beams to rapidly analyze individual particles — can be adapted to identify and count microplastic and nanoplastic particles, potentially offering a faster and more automated approach to pollution monitoring.
Flow cytometry is a technique that uses a beam of light to simultaneously measure and analyze the physical characteristics of single particles (usually cells) as they flow in a fluid stream. These characteristics can be determined using an optical-to-electronic...
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Micro and Nano Plastics: Identification and Quantification Methods
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Researchers reviewed the primary identification and quantification methods for micro and nanoplastics (MNPs), covering spectroscopic, microscopic, and chemical analytical techniques used to detect particles across environmental and biological matrices. Standardized, reliable detection methods are essential for accurately tracking MNP contamination levels and informing regulatory and public health responses.
Detection and characterization of microplastics and nanoplastics in biological samples
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Researchers reviewed current methods for detecting and characterizing microplastics and nanoplastics in biological samples, finding that most techniques are optimized for environmental media like water and perform poorly in complex biological matrices, and recommending improved workflows for digestion, separation, enrichment, and analysis of particles in organisms and human tissues.
Fluorescence imaging of microplastics and nanoplastics in biological samples
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Researchers reviewed fluorescence imaging techniques for detecting microplastics and nanoplastics in biological samples, highlighting advances in fluorescent dye staining, label-free autofluorescence detection, and super-resolution microscopy that can visualize particles as small as 50 nm inside living organisms.
Challenges in the Analysis of Micro- and Nanoplastics
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Identifying the chemical composition of micro- and nanoplastics using techniques such as spectroscopy and chromatography is critical for understanding their environmental fate and toxicological risks. Overcoming current analytical challenges in detecting nanoscale plastic particles remains a key barrier to comprehensive risk assessment.
Flow cytometry as new promising detection tool for micro and submicron plastic particles
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Researchers evaluated flow cytometry as a tool for detecting and counting micro- and submicron plastic particles in environmental and biological samples. The method offered rapid throughput and the ability to distinguish plastic particles from biological material, but required careful optimization for complex matrices.
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