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Interaction of Rhodamine 6G with Biodegradable Poly(lactic Acid) Nanoparticles: A Suitable Analytical Strategy for the Detection of Nanoplastics in Water
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Researchers found that poly(lactic acid) nanoparticles (70 nm) quench rhodamine 6G fluorescence via static complex formation in the ground state, with Stern-Volmer sensitivity increasing 1.88-fold over 5 minutes due to conformational changes, enabling a sensitive analytical method for detecting nanoplastics in aqueous systems.
Poly(lactic acid) nanoparticles (PLA-NP) with an average size of 70 nm, effectively quench the fluorescence emission of rhodamine 6G via static quenching. This process occurs as a result of forming a nonfluorescent complex in the ground state. The determined Stern–Volmer constant depends on the incubation time, being 1.88 times more sensitive at 5 min compared to 1 min, attributed to conformational changes after the rhodamine binds to the nanoparticle surface. The decrease in the fluorescence signal was employed as the analytical strategy for detecting and quantifying PLA-NP in aqueous systems, achieving a limit of detection of 26.5 pM. The accuracy of the determination was demonstrated with 95% confidence by recovery assays (90%–110%) in spiked samples of surface river water. This method represents a promising tool for researchers and environmental scientists dedicated to monitoring and mitigating water pollution caused by nanoplastics.
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Interaction of Rhodamine6G with Biodegradable Poly(lacticAcid) Nanoparticles: A Suitable Analytical Strategy for the Detectionof Nanoplastics in Water
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Researchers found that poly(lactic acid) nanoparticles (70 nm average size) quench rhodamine 6G fluorescence through static quenching via ground-state complex formation, with sensitivity improving 1.88-fold at 5 minutes versus 1 minute incubation, providing a quantitative fluorescence-based strategy for detecting nanoplastics in water.
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