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Data for publication: WHEN FLUORESCENCE IS NOT A PARTICLE: THE TISSUE TRANSLOCATION OF MICROPLASTICS IN DAPHNIA MAGNA SEEMS AN ARTEFACT
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This dataset accompanies a replication study investigating whether the apparent translocation of microplastics into the tissues of Daphnia magna (a tiny water flea) was a real biological phenomenon or an artifact of fluorescence microscopy. The study raises important methodological cautions for researchers using fluorescent particles to track microplastic uptake in organisms.
We conducted a replication study for Rosenkranz et al. (2009).Our data consists of CLSM images of Daphnia exposed to 20 nm and 1000 nm polystyrene particles with a number of conditions and specimen treatments.
More Papers Like This
Data for publication: WHEN FLUORESCENCE IS NOT A PARTICLE: THE TISSUE TRANSLOCATION OF MICROPLASTICS IN DAPHNIA MAGNA SEEMS AN ARTIFACT
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Researchers conducted a replication study and found that apparent tissue translocation of microplastics in the water flea Daphnia magna was likely a fluorescence artifact rather than true uptake — an important methodological finding that calls into question some earlier studies reporting microplastic internalization in small organisms.
When Fluorescence Is not a Particle: The Tissue Translocation of Microplastics in Daphnia magna Seems an Artifact
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Researchers investigated whether fluorescent polystyrene microplastic beads truly translocate from the gut to body tissues in the water flea Daphnia magna, as previous studies had reported. Using confocal microscopy and passive sampling, they found that the fluorescent dye leached off the plastic beads and accumulated in lipid droplets independently of the particles themselves. The study demonstrates that using fluorescence as a proxy for tracking microplastics can produce misleading results about tissue uptake.
The translocation of microplastics to lipid droplets of Daphnia magna is an artefact
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This study attempted to replicate previous findings of polystyrene nanoparticle translocation from the gut to lipid droplets in Daphnia magna, but found that the observed fluorescence in lipid droplets was caused by dye leaching from the plastic particles rather than actual particle translocation. The findings highlight the importance of rigorous controls and skepticism in microplastic uptake research.
Daphnia magna uptake and excretion of luminescence‐labelled polystyrene nanoparticle as visualized by high sensitivity real-time optical imaging
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Researchers used lanthanide-labeled polystyrene nanoparticles and time-gated imaging to track nanoplastic uptake and excretion in live Daphnia magna in real time, finding that the water flea accumulated roughly 12 nanograms of nanoplastics per individual after 24 hours and that a portion migrated into the circulatory system before being slowly excreted over 48 hours.
Selective ingestion and response by Daphnia magna to environmental challenges of microplastics
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Researchers used fluorescent microplastics labeled with aggregation-induced emission markers to investigate how Daphnia magna selectively ingests different types of plastic particles, finding that particle type, size, and surface chemistry influence ingestion patterns and toxicological response.
Research digests by email
When a large batch of papers lands in the Atlas, we read through it and send a short write-up of what stood out.