(ESM - SUBMITTED AS ONE FILE) Supplemental Figures 1 - 3 and Supplemental Tables 1 - 3 from Patterns, dynamics and consequences of microplastic ingestion by the temperate coral, <i>Astrangia poculata</i>

Figure S1: Confocal (Zeiss AiryScan) Imaging of GFP+<i>E. coli</i>-biofilmed microbead. Scan of one hemisphere of the opaque microbeads demonstrates <i>E. coli</i> (green) cells fully coating the bead.; Figure S2: Particle consumption of both microplastics (MP) and brine shrimp eggs (BSE) is not correlated with polyp volume.; Figure S3. <i>Astrangia poculata</i> mortality following ingestion of GFP+ E. coli-biofilmed microbeads (Treatment) and non-biofilmed microbeads (Control). Death of the polyps that were directly fed (=“focal polyp”) and the polyps surrounding the focal polyps (=“neighboring polyps”) were scored for mortality during timepoints over 28 days. N = 6 treatment coral colonies (fed microbeads incubated in GFP + E. coli in LB broth); N = 6 control coral colonies (fed microbeads incubated in LB broth only). In each colony, 3 focal polyps and all neighboring polyps were scored.; Table S1. Results from paired permutation tests in R on percentages of beads contained in different portions of individual polyps. NS = not significant.; Table S2. Average microplastic bead consumption rates observed at all experimental time points.; Table S3. Results from unpaired permutation-tests in R comparing total beads consumed per polyp during different time points. NS = not significant. Time point with more beads per polyp also denoted for comparisons with significant differences.