Acute and chronic effects of polystyrene microplastics on juvenile and adult Daphnia magna

We investigate the distribution and effects of polystyrene microplastic (MP) particles in exposure experiments with the ecotoxicology model organism Daphnia magna. The aim was to investigate the short and long-term toxicity of MP at different concentrations. To achieve this goal, the effects of 6 μm commercially available polystyrene beads on two different life-stages of D. magna: < 24 h old juveniles and 9 days old adults was assessed. The following end points in test animals were measured: (1) survival, (2) growth, (3) individual and population fecundity, (4) age at maturation and (5) body size of newborn offspring. These response variables were followed in two acute and two chronic experiments. The acute experiments showed that MP is not acutely toxic to D. magna within 48 h, but cause added mortality within 120 h. The juveniles were about 50% more sensitive than the adults tested. In life-cycle experiments testing chronic exposure to MP, again, animals exposed as juveniles at relatively high concentrations, i.e. > 30 μg ml showed higher sensitivity. We observed slightly increased mortality, reduced growth and stimulation of early reproduction at the cost of later reproduction. Animals exposed after reaching adulthood did not show increased mortality and showed a stimulation response with higher reproductive rates than the control group. However, both the growth rate of mother animals and the body size of newborn declined with increasing dose of MP. We conclude that these effects indicate a role of MP in mechanical interaction/interference with the animal on the level of feeding (clogging filtering functions), digestion (gut filled with plastic particles), and/or other animal behavior. The study also illustrates how MP with slow break-down rates may accumulate in the environment and enter the food-chain as obstructing non-food particles in filter-feeding organisms.