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Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Frontiers in Environmental Science 2019 47 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Annika Batel, Annika Batel, Annika Batel, Xavier Cousin, Thomas Braunbeck, Annika Batel, Annika Batel, Bettie Cormier, Bettie Cormier, Bettie Cormier, Bettie Cormier, Bettie Cormier, Jérôme Cachot, Annika Batel, Bettie Cormier, Annika Batel, Annika Batel, Annika Batel, Annika Batel, Annika Batel, Steffen Keiter Annika Batel, Jérôme Cachot, Jérôme Cachot, Bettie Cormier, Annika Batel, Thomas Braunbeck, Annika Batel, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Xavier Cousin, Jérôme Cachot, Jérôme Cachot, Bettie Cormier, Steffen Keiter Marie‐Laure Bégout, Steffen Keiter Steffen Keiter Jérôme Cachot, Jérôme Cachot, Steffen Keiter Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Xavier Cousin, Jérôme Cachot, Jérôme Cachot, Bettie Cormier, Bettie Cormier, Steffen Keiter Bettie Cormier, Annika Batel, Thomas Braunbeck, Steffen Keiter Annika Batel, Marie‐Laure Bégout, Jérôme Cachot, Steffen Keiter Jérôme Cachot, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Xavier Cousin, Steffen Keiter Xavier Cousin, Steffen Keiter Marie‐Laure Bégout, Jérôme Cachot, Steffen Keiter Xavier Cousin, Marie‐Laure Bégout, Jérôme Cachot, Steffen Keiter Steffen Keiter Jérôme Cachot, Marie‐Laure Bégout, Marie‐Laure Bégout, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Marie‐Laure Bégout, Marie‐Laure Bégout, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Xavier Cousin, Steffen Keiter Steffen Keiter Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Thomas Braunbeck, Annika Batel, Annika Batel, Annika Batel, Annika Batel, Xavier Cousin, Steffen Keiter Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Marie‐Laure Bégout, Jérôme Cachot, Bettie Cormier, Bettie Cormier, Steffen Keiter Steffen Keiter Xavier Cousin, Jérôme Cachot, Marie‐Laure Bégout, Jérôme Cachot, Xavier Cousin, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Xavier Cousin, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Steffen Keiter Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Steffen Keiter Steffen Keiter Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Steffen Keiter Jérôme Cachot, Marie‐Laure Bégout, Steffen Keiter Jérôme Cachot, Jérôme Cachot, Marie‐Laure Bégout, Jérôme Cachot, Xavier Cousin, Xavier Cousin, Jérôme Cachot, Jérôme Cachot, Marie‐Laure Bégout, Thomas Braunbeck, Jérôme Cachot, Jérôme Cachot, Jérôme Cachot, Xavier Cousin, Xavier Cousin, Xavier Cousin, Marie‐Laure Bégout, Xavier Cousin, Marie‐Laure Bégout, Marie‐Laure Bégout, Marie‐Laure Bégout, Jérôme Cachot, Xavier Cousin, Jérôme Cachot, Xavier Cousin, Bettie Cormier, Bettie Cormier, Xavier Cousin, Jérôme Cachot, Xavier Cousin, Xavier Cousin, Xavier Cousin, Marie‐Laure Bégout, Jérôme Cachot, Steffen Keiter Jérôme Cachot, Jérôme Cachot, Steffen Keiter

Summary

A multi-laboratory study using an enhanced zebrafish embryo test assessed the hazard of microplastics contaminated with environmental pollutants, finding that the combination of plastics and sorbed chemicals caused greater toxicity than either alone. The inter-laboratory approach helps validate this testing method for regulatory use in assessing microplastic hazards.

Polymers

Body Systems

Reproductive

Models

Zebrafish

As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP) and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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