Hepatotoxicity of pristine polyethylene microplastics in neotropical physalaemus cuvieri tadpoles (Fitzinger, 1826)

Plastic waste disposal in the environment is a major issue worldwide, whose effects on different biotas are the object of several investigations. The toxicity caused by microplastics (MPs) in organisms living in freshwater environments remains little explored. Little is known about the consequences of the exposure to these pollutants on the health of amphibians. Thus, we tested the hypothesis that the exposure of Physalaemus cuvieri tadpoles to microplastic polyethylene (PE MP) causes histopathological damage to their liver. Data collected after seven days of exposure to MPs (60 mg/L) have shown that pollutant bioaccumulation in tadpoles' liver was correlated to different histopathological changes (blood vessel dilation, infiltration, congestion, hydropic degeneration, hypertrophy and hyperplasia), which showed the histopathotoxicity of MPs. Furthermore, we observed changes in hepatocyte nuclei size (area and diameter), volume and shape induced by the exposure to PE MPs, a fact that evidenced the cytotoxic effect of these pollutants. To the best of our knowledge, the current study is the first to report the histopathotoxicity of PE MPs in representatives of the amphibian group, and it contributes to improving knowledge about these pollutants and how they may affect the health of these animals.