Is the development of <i>Daphnia magna</i> neonates affected by short-term exposure to polyethylene microplastics?

The study presents responses of <i>D. magna</i> newborns exposed during 96 h to polyethylene microplastics (MP) of size 40-48 μm in the concentrations of 20; 40; 80; 160 and 320 mg/L. The experimental design consisted of two exposure scenarios: the first group was fed at the beginning and after 48 h (3x10^-5 cells/mL of <i>Raphidocelis subcaptata</i> and fermented solution) and the second group was not fed as an additional stressor. The mobility of the organisms was not significantly affected in the presence of microplastics for both exposure groups. Nevertheless, the qualitative analysis showed that neonates promptly ingested microplastics in the first 24 h of the test, independently of the treatment. Polyethylene microplastics did not influence the molting process, however, significant differences were observed between the number of molts of the exposure without feed and with feed in 24 h (p = 0.0007), 48 h (p = 2.4 x 10^-10), 72 h (p = 3.6 x 10^-10) and 96 h (p = 0.003). The final body length of <i>D. magna</i> also showed that the food administration model in the tests contributes to the differentiation in responses.