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Quantification of polystyrene plastics degradation using 14C isotope tracer technique

Methods in enzymology on CD-ROM/Methods in enzymology 2021 12 citations

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Researchers described how carbon isotope tracing can be used to quantitatively track the degradation of polystyrene plastics in complex environmental samples. This technique can help researchers understand how slowly conventional plastics break down in the environment and what breakdown products they produce.

Polymers

C-isotope tracer technique is an effective tool for quantitative analysis of compounds. Based on its unique C signal, C-isotope tracer has been widely used in degradation of pollutants, especially in a complex environmental matrix. In this chapter, we introduce methods and examples for studying the degradation of polystyrene (PS) plastics using the C-isotope tracer technique. C-based gel permeation chromatography (GPC), high performance liquid chromatography (HPLC) as well as liquid scintillation counter (LSC) are introduced for analyzing changes in the molecular weight of plastic polymers, generation of hydrophilic products, and complete mineralization of plastics during microbial and UV-induced degradation. With the C-isotope tracer technique, further studies on mechanisms for degradation of plastics/microplastics under complex natural environment conditions could be facilitated.

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