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Immobilization of PETase enzymes on magnetic iron oxide nanoparticles for the decomposition of microplastic PET

Nanoscale Advances 2021 96 citations ? Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count. Score: 45 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.

Sebastian P. Schwaminger, Sebastian P. Schwaminger, Sebastian P. Schwaminger, Stefan Fehn, Tobias Steegmüller, Stefan Rauwolf, Hannes Löwe, Katharina Pflüger‐Grau, Sonja Berensmeier

Summary

PETase enzymes capable of degrading polyethylene terephthalate (PET) were immobilized on superparamagnetic iron oxide nanoparticles via a His-tag to improve stability and enable magnetic recovery. The immobilized enzyme system showed enhanced efficiency and reusability for breaking down PET microplastics in water.

Polymers

PE PET

Polyethylene terephthalate (PET) is responsible for a large amount of environmental contamination with microplastics. Based on its high affinity, the PET degrading enzyme PETase can be immobilized on superparamagnetic iron oxide nanoparticles through a His-tag. The His-tag increases enzyme stability, and allows magnetic separation for recovery. Multiple recycling steps are possible and microplastic particles can be decomposed depending on the PET's crystallinity. The separation or decomposition of PET allows for a sustainable way to remove microplastic from water.

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