Microplastics disrupt accurate soil organic carbon measurement based on chemical oxidation method

Microplastics are widespread contaminants in soils and terrestrial ecosystems in many areas worldwide. In this study, we measured soil organic carbon (SOC) and soil organic matter (SOM) in microplastic-treated soils to determine if the presence of microplastics could affect the accuracy of carbon-based soil quality indicator measurements. Six different sizes and types of microplastics were selected, and six soil samples were used to evaluate the impacts. Treating soil with polyethylene and low-density polyethylene significantly increased SOC (p < 0.05) when measured with the modified Walkley & Black method; microplastic addition (0.01%, v/v) increased SOC by >40% compared to control organic carbon-poor soil (<10.0 g kg). We conclude that the microplastics can disrupt the accurate measurement of SOC. Likely, the physicochemical treatment used in the SOC measurement process can cause the organic compounds and/or carbon complexes to be extracted from microplastics, and this can affect the results. Considering that SOC is a main indicator for assessing soil quality and the global carbon cycle, overestimations caused by microplastic contamination should be further discussed to identify appropriate ways to deal with microplastics as a new carbon source in the environment.