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Polyethylene Terepthalate (PET) biodegradation and characterisation of landfill leachate bacteria

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This Icelandic undergraduate thesis reviews PET plastic biodegradation mechanisms and characterizes bacteria isolated from landfill leachate for their ability to degrade PET. The work explores biological pathways for breaking down one of the most widely used plastics, which otherwise accumulates as persistent microplastic pollution.

Polymers

Í þessu verki er fjallað um framleiðslu og byggingu plastfjölliða og áhrif þeirra á umhverfið eftir notkun. Lífniðurbrot á plasti kynnt og helstu bakteríum og ensímum lýst með áherslu á pólýetýlen terephthalate (PET). Horft á framtíðarmöguleika á þessu sviði. Einnig eru eiginleikar baktería sem einangraðar hafa verið úr affalsvatni sorpurðunarstöðvar skoðaðar með tilliti til niðurbrots á plastefnum. Í ljósi þess að töluvert magn af PET hefur verið fargað á urðunarstaðnum á notkunarárum þess, má alykta að aðgengi baktería að PET í nærumhverfinu hafi leitt til genatjáningar fyrir niðurbroti þess. Bakteríur voru prófaðar fyrir þekjumyndun á yfirborði plasts, framleiðslu yfirborðsvirkra efna og ensímvirkni. Bakteríur voru einnig prófaðar fyrir tilvist tveggja þekktra PETasa(p-Nitrobenzylesterasi og Carboxylesterasi) með PCR tækni. Rannsóknarspurning: Eru bakteríur sem framleiða PETasa meðal þeirra sem einangruð hafa verið frá Sigurðargilslæk?

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