Size-dependent transgenerational toxicity induced by nanoplastics in nematode Caenorhabditis elegans

Nanoplastic exposure can potentially cause the severe transgenerational toxicity in organisms. However, the transgenerational nanoplastic toxicity and the underlying mechanisms are still largely unclear. Using Caenorhabditis elegans as an animal model, we here compared the transgenerational toxicity of two sizes of polystyrene nanoparticles (PS-NPs, 20 and 100 nm). The nematodes were exposed to PS-NPs at the P0 generation, and from the F1 generation the nematodes were grown under the normal condition. Exposure to 20 nm PS-NPs resulted in more severe transgenerational toxicity than exposure to 100 nm PS-NPs. At the concentration of 100 μg/L, the toxicity of 20 nm PS-NPs on locomotion and reproduction was detected at the F1-F6 generations, whereas the toxicity of 100 nm PS-NPs could only be observed at the F1-F3 generations. The difference in transgeneration toxicity between PS-NPs (20 nm) and PS-NPs (100 nm) was associated with the difference in transgenerational activation of oxidative stress. Based on observations on SOD-3::GFP, HSP-6::GFP, and HSP-4::GFP expressions, PS-NPs (20 nm) and PS-NPs (100 nm) further induced different transgenerational responses of anti-oxidation, mt UPR, and ER UPR. Our data suggested that the induction of transgenerational toxicity of PS-NPs was size dependent in nematodes. The results are helpful for our understanding the cellular mechanisms for the induction of transgenerational nanoplastic toxicity in organisms.