Evaluation of different packaging methods and storage temperature on MPs abundance and fillet quality of rainbow trout

There are many studies on microplastics (MPs) about the aquatic ecosystems and its components. However, there is limited study on the MPs abundance, identification and sources in processed seafood products which are manufactured for direct human consumption. In this study, rainbow trout (Oncorhynchus mykiss) fillets were packed with different packaging techniques and stored at two different temperatures (+4 and -20°C) for 21 days. The presence, shape, size and polymer type of MPs were determined by ATR-FTIR on certain days (7, 14 and 21 days) in fillets during storage. The chemical quality changes in fillets [with pH, thiobarbituric acid reactive substrate (TBARS), and total volatile basic nitrogen (TVB-N) data] were monitored and the effect of MPs presence was evaluated. At the last step, the estimated MPs intake level in humans was determined with considering the presence of MPs (determined in fillets). The presence of MPs was determined the most in the Polystyrene plate + wrapped film (S) group and the least in the Chitosan film + Polystyrene plate + wrapped film (C) group. When evaluated in terms of chemical parameters, although good results were obtained in all samples stored at - 20°C, the presence of MPs was determined at a high level in fillets which stored at this temperature. As a result of the study, it was determined that the packaging type and storage temperature have significant effects on the presence of MPs and fillet quality.