Article
?
AI-assigned paper type based on the abstract. Classification may not be perfect — flag errors using the feedback button.
Tier 2
?
Original research — experimental, observational, or case-control study. Direct primary evidence.
Environmental Sources
Human Health Effects
Sign in to save
Assessing the presence of microplastic particles in Tunisian agriculture soils and their potential toxicity effects using Eisenia andrei as bioindicator
The Science of The Total Environment2021
122 citations
?
Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 60
?
0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Researchers investigated microplastic contamination in Tunisian agricultural soils under different farming practices, including organic farming, greenhouses, mulching, and wastewater irrigation. They found that earthworms readily ingested the microplastics, with particles from mulched and wastewater-irrigated soils causing significant oxidative stress in the worms. The study provides new evidence that agricultural microplastics pose a real threat to soil-dwelling organisms and terrestrial ecosystems.
In the present study, we investigated microplastics (MPs) in agricultural soils with different agronomic practices (organic farming, soil under greenhouses, soil under mulching and soil irrigated with treated wastewater (TWW)). Plastic particles from each site were collected and characterized by FTIR and Raman microspectroscopy. Plastic particles were then ground and added (size rage under 100 μm) at a concentration of 100 μg kg to soils from organic farming containing Eisenia andrei for 7 and 14 days. MPs accumulation in earthworms was quantified and characterized. Oxidative stress was assessed by evaluating the activities of catalase, glutathione-S-transferase, and acetylcholinesterase as well as malondialdehyde accumulation. Our results revealed higher quantity of MPs in soils ranging from 13.21 ± 0.89 to 852.24 ± 124.2 items kg with the dominance of small sizes (0.22-1.22 μm). Polyethylene (PE) and polybutyrate adipate terephtalate (PBAT) were the dominant MPs. Moreover, our results revealed a significant ingestion of MPs in earthworms with values ranging from 1.13 to 35.6, characterized mostly by PE, PBAT and polypropylene (PP). Biochemical data revealed an important alteration in worms exposed to MPs from soils with mulching and irrigated with TWW. Our study provides new insights into the effects of microplastic in earthworms and thus the vulnerability of terrestrial ecosystem to this emergent contaminant.