Influence of polystyrene microplastics on levofloxacin removal by microalgae from freshwater aquaculture wastewater

Chlorella vulgaris (C. vulgaris) has attracted widespread attention because of its ability to absorb, enrich, and degrade typical endocrine-disrupting antibiotics (such as levofloxacin) in aquaculture wastewater. However, microplastic pollution in wastewater, which is becoming an increasingly severe problem, will exert a toxic effect on aquatic organisms (such as C. vulgaris and other microalgae). Polystyrene microplastics (PS-MPs), which are commonly found in freshwater aquaculture wastewater, are the most harmful. Therefore, clarifying the effects of PS-MPs on the ability of C. vulgaris to degrade typical endocrine-disrupting antibiotics in freshwater aquaculture wastewater and determining the mechanism of the effect are particularly important. The results of this study showed that under the stress of PS-MPs, the growth of C. vulgaris was significantly inhibited; the EPS-polysaccharide content per algal cell, EPS adsorption, intracellular enrichment and degradation of levofloxacin, total CYP450 content, and total CYP450 activity all decreased; and the relative expression of key genes related to the metabolic activity of algal cells, such as psbA, psaB, and rbcL, was generally downregulated. PS-MPs mainly affected the removal of a typical endocrine-disrupting antibiotic by C. vulgaris by altering adsorption, enrichment, and enzyme degradation. The results provide a reference for research on the impact of microplastic pollution on the treatment of freshwater aquaculture wastewater.