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Multi-level pie chart demonstrating the proportion of microplastics found in gastric lavage and GI samples.
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This supplementary figure presents a multi-level pie chart showing the proportional distribution of microplastic shapes recovered from gastric lavage samples versus dissected gastrointestinal tracts in cowbirds, visually comparing the two collection methods.
Blue sections represent microplastics recovered from lavage and red sections represent microplastics detected in the gastrointestinal tracts in cowbirds, broken down by microplastic shape. Created with biorender.com.
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Visual comparison of microplastic counts in lavage and gastrointestinal tract samples of cowbirds.
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Researchers compared microplastic counts recovered from gastric lavage samples against those found in dissected gastrointestinal tracts of cowbirds, demonstrating the relationship between lavage recovery rates and total microplastic burden per individual bird.
Brown-headed Cowbird paired lavage and GI microplastics data (April-May 2023)
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Researchers used gastric lavage as a non-lethal method to sample microplastic ingestion in 105 wild Brown-headed Cowbirds in Texas, documenting MP types and quantities in the gizzard contents and validating this sampling technique for monitoring plastic pollution in free-living birds.
Gastric lavage may not be representative of total microplastic ingestion for a wild passerine bird
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Researchers tested non-lethal gastric lavage in 105 Brown-headed Cowbirds and found it recovered only an average of 50.4% of total ingested microplastics — with wide individual variability (0–100%) — concluding the technique provides unreliable estimates of total microplastic body burden.
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Researchers examined microplastic in the gastrointestinal tracts of 143 Northern Fulmars and 25 Sooty Shearwaters stranded on Oregon and Washington beaches, finding plastic in 89.5% and 64% of birds respectively, with larger particles concentrated in the stomach's upper chamber and no significant reduction in plastic load in birds held in a plastic-free rehabilitation environment for up to seven days.
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