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Application Of Spectrochemical Analysis with Chemometrics to Profile Biochemical Alterations in Nanoplastics-Exposed Hepg2 Cells
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Probing Cytotoxic and Oxidative Stress Effects of Nanoplastics on Human Intestinal Caco-2 Cells: Insights from Raman Spectroscopy and Machine Learning
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Scientists exposed human intestinal cells to tiny plastic particles (nanoplastics) in a lab dish and found that they caused cell damage and stress, with higher amounts of plastic causing more harm. Using a laser-based technique combined with computer analysis, researchers could detect specific chemical changes in the cells' fats and proteins caused by the plastic exposure, offering a new way to track how these ubiquitous pollutants might affect our gut cells. Since we regularly ingest microplastics and nanoplastics through food, water, and packaging, this research helps build the case that these particles could pose real risks to digestive health
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Researchers reviewed how nanoplastics accumulate in and harm living organisms, finding that particle size, surface charge, exposure dose, and duration all influence toxicity — with smaller, charged particles penetrating cells more readily and chemical additives amplifying harm beyond the plastics themselves.
Application of spectrochemical analysis with chemometrics to profile biochemical alterations in nanoplastic-exposed HepG2 cells
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Researchers used infrared and Raman spectroscopy to profile biochemical changes in human liver cells (HepG2) exposed to nanoplastics with different surface charges at low environmental concentrations, finding that amine-functionalized nanoplastics caused the most toxicity and inhibited cell proliferation, while carboxyl-functionalized particles unexpectedly induced DNA damage.
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Researchers reviewed evidence from cell and animal studies on the toxic effects of micro- and nanoplastics, finding that these particles can cause inflammation, oxidative stress, and organ damage in laboratory models, raising concern about what chronic low-level human exposure might mean for long-term health.
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