Effects of microplastics of different sizes on the Chlorella vulgaris - Ganoderma lucidum co-pellets formation processes

The effects of different sized MPs on the formation process of algal-fungal co-pellets were studied. The results show that a maximum biomass recovery of 70.96% and a minimum F/F ratio of 0.463 reached with 5.000 μm-microplastics. Chlorella vulgaris cells and microplastics adhered evenly to the mycelia of Ganoderma lucidum. The contact angle decreased 24.02% and 34.68% with addition of 0.065 μm and 0.500 μm microplastics, respectively, compared to the control group, while the lowest crystallinity index (7.05%) was obtained with 0.065 μm-microplastics addition. Moreover, 5.000 μm microplastics promoted the extracellular polymeric substances (EPS) secretion, with the soluble polysaccharide content increasing by 40.50% and the soluble protein content increasing by 23.25% compared with the single algal-fungal system, while bound polysaccharides increased by 113.26% and bound proteins increased by 29.48%. The 5.000 μm microplastics also significantly promoted enzyme activity in the co-pellets. These results provide a theoretical basis for algal recovery in microplastic-containing water.