Combined effects of oxytetracycline and microplastic on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles

The widespread application of antibiotics and plastic films in agriculture leads to new characteristics of soil pollution with the coexistence of antibiotics and microplastics. However, their combined effects on wheat seedling growth and associated rhizosphere bacterial communities and soil metabolite profiles remain unclear. Here, in the potted experiment, wheat was treated with individual oxytetracycline (0, 5.0, 50.0, and 150.0 mg kg) and the combination of oxytetracycline and polyethylene microplastic (0.2%). Results showed that 150 mg kg oxytetracycline combined with microplastic significantly reduced the biomass and height of the plant. Compared with CK, all the treatments exposed to the combination of oxytetracycline and polyethylene microplastic significantly promoted carotenoid content and peroxidase activity in wheat leaves. Soil dehydrogenase and urease activities were more sensitive to current pollutant exposure than sucrase activity. Oxytetracycline (150 mg kg) alone and in combination with polyethylene significantly decreased the abundances of certain genera belonging to plant growth-promoting rhizobacteria (PGPR) in soil, such as Arthrobacter, Gemmatimonas, Massilia, and Sphingomonas. Combined exposure of 150 mg kg oxytetracycline and polyethylene microplastic significantly altered multiple metabolites including organic acids and sugars. Network analysis indicated that co-exposure of 150 mg kg oxytetracycline and microplastic may affect the colonization and succession of PGPR by regulating soil metabolites, thereby indirectly inhibiting wheat seedling growth. The results help to elucidate the potential mechanisms of phytotoxicity of the combination of oxytetracycline and polyethylene microplastic.