Peer Review #2 of "Microplastics do not affect bleaching of Acropora cervicornis at ambient or elevated temperatures (v0.2)"

Microplastic pollution can harm organisms and ecosystems such as coral reefs.Corals are important habitat-forming organisms that are sensitive to environmental conditions and have been declining due to stressors associated with climate change.Despite their ecological importance, it is unclear how corals may be affected by microplastics or if there are synergistic effects with rising ocean temperatures.To address this research gap, we experimentally examined the combined effects of environmentally relevant microplastic concentrations (i.e., the global average) and elevated temperatures on bleaching of the threatened Caribbean coral, Acropora cervicornis.In a controlled laboratory setting, we exposed coral fragments to orthogonally crossed treatment levels of low-density polyethylene microplastic beads (0 and 11.8 particles L -1 ) and water temperatures (ambient at 28°C and elevated at 32°C).Zooxanthellae densities were quantified after the 17-day experiment to measure the bleaching response.Regardless of microplastic treatment level, corals in the elevated temperature treatment were visibly bleached and necrotic (i.e., significant negative effect on zooxanthellae density) while those exposed to ambient temperature remained healthy.Thus, our study successfully elicited the expected bleaching response to a high-water temperature.However, we did not observe significant effects of microplastics at either individual (ambient temperature) or combined levels (elevated temperature).Although elevated temperatures remain a larger threat to corals, responses to microplastics are complex and may vary based on focal organisms or on plastic conditions (e.g., concentration, size, shape).Our findings add to a small but growing body of research on the effects of microplastics on corals, but further work is warranted in this emerging field to fully understand how sensitive ecosystems are affected by this pollutant.