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Microplastics in the Tentacles and Digestive Tract of Squid from TPI Tambak Lorok
Original title: Mikroplastik pada Tentakel dan Pencernaan Cumi – Cumi dari TPI Tambak Lorok
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This Indonesian-language study examined microplastic contamination in the tentacles and digestive systems of squid from a fish landing site in Semarang, documenting the presence of microplastics in this commercially important seafood. The findings add to evidence that microplastic ingestion by cephalopods is occurring and may represent a pathway for human dietary exposure.
TPI Tambak Lorok adalah salah satu TPI yang ada di Semarang. Beberapa biota laut didaratkan di TPI tersebut salah satunya cumi–cumi. Penelitian cumi–cumi masih jarang dilakukan, seperti penelitian mengenai mikroplastik pada cumi – cumi, dikarenakan cumi–cumi merupakan salah satu biota konsumsi maka perlu diketahui apakah mikroplastik sudah masuk kedalam cumi – cumi. Penelitian ini bertujuan mengetahui jumlah, bentuk, warna dan ukuran mikroplastik pada tentakel dan pencernaan cumi- cumi dari TPI Tambak Lorok. Penelitian dilakukan bulan Juli–November 2020 di Semarang. Pengambilan sampel dilakukan di TPI Tambak Lorok. Cumi – cumi yang didapatkan diukur menggunakan jangka sorong. Selanjutnya sampel dibedah menggunakan gunting dipisahkan tentakel dan pencernaannya. Pelarutan bahan organik menggunakan KOH 10% selama 24 jam. Pemisahan partikel mikroplastik menggunakan ZnCl2 selama 24 jam. Pemisahan partikel akan membentuk 2 lapisan yaitu cairan bening dan endapan. Cairan bening diambil 10 ml untuk dilakukan penyaringan menggunakan vacum pump. Hasil vacum pump dikeringkan dilanjutkan pengamatan visual menggunakan mikroskop untuk dihitung jumlah, diamati bentuk, warna dan ukuran mikroplastik. Mikroplastik ditemukan lebih banyak pada pencernaan 343 partikel dan pada tentakel 212 partikel. Bentuk mikroplastik yang ditemukan adalah fiber, film, fragmen dan pelet. Mikroplastik ditemukan 6 warna yaitu hitam, putih, merah, oranye, kuning, biru. Ukuran mikroplastik ditemukan 0,54–100,75 µm. TPI Tambak Lorok is one of the TPI in Semarang. Some marine biota landed at the TPI, one of which was squid. Research on squid is still rarely done, such as research on microplastics in squid, because squid is one of the consumption biota, it is necessary to know whether microplastics have entered the squid. The purpose of this research is to determine the total, shape, color and size of microplastics in the tentacles and digestion of squid from TPI Tambak Lorok. The research was conducted in July 2020 – November 2020 in Semarang. Sampling was carried out at TPI Tambak Lorok. The squid obtained were measured using a caliper. Next step, the sample was dissected using scissors to separate the tentacles and digestive tract. Dissolution of organic ingredients using 10% KOH for 24 hours. Separation of microplastic particles using ZnCl2 for 24 hours. Separation of particles will form 2 layers, namely clear liquid and precipitate. 10 ml of clear liquid was taken to be filtered using a vacuum pump. The results of the vacuum pump were dried followed by visual observations using a microscope to count the total, observe the shape, color and size of microplastics. Microplastics were found more in the squid digestion of 343 particles and in the squid tentacles of 212 particles. The shape of microplastics found were fiber, film, fragments and pellets. Microplastics were found in 6 colors that is black, white, red, orange
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