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Tara Mission Microplastics rDNA 18S V9 ASV table (DADA2)
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Researchers released an 18S V9 amplicon sequence variant (ASV) table generated via DADA2 from the Tara Mission Microplastics expedition, providing a eukaryotic community dataset linked to microplastic-associated samples across global ocean transects. The dataset includes contextual metadata on sampling protocols, temperature, and nutrient concentrations to facilitate ecological analysis of plastisphere communities.
Description This repository contains the rDNA 18S V9 ASV table (DADA2) for Tara Mission Microplastics built with nf-core/ampliseq. Files tara-plastic_18s-v9_ampliseq_report.html: nf-core/ampliseq report detailing each bioinformatic step. tara-plastic_18s-v9_context.tsv.gz: Contextual data table with 25 tab separated fields. For each sample, information such as the sampling protocol, temperature and nutrients concentration can be found. tara-plastic_18s-v9_dada2_phyloseq.rds: ASV table in phyloseq format, see below for more details about the ASV table. tara-plastic_18s-v9_dada2_table.tsv.gz: ASV table with 269 tab separated fields. ASV are identified by the md5 (asv_id) of their sequence, and were taxonomically assigned (domain to species) with dada2's assignTaxonomy() using PR2 5.0.0. The per sample read numbers for each ASV (n=82,612) are indicated in the columns from I_LOIRE_S03_S325-T_PA to G_TIBRE_S01_CG_POM). Each sample name is composed as followed {RIVER_CODE}_{RIVER_NAME}_{STATION_NUMBER}_{PROTOCOL}_{SAMPLE_TYPE}. ASV table assembly The ASV table has been assembled with nf-core/ampliseq version 2.7.1. More details can be found in the ampliseq report (tara-plastic_18s-v9_ampliseq_report.html) and all the scripts are gathered in this gitlab repository
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Tara Mission Microplastics rDNA 18S V9 ASV table (DADA2)
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Researchers released an 18S V9 amplicon sequence variant (ASV) table generated via DADA2 from the Tara Mission Microplastics expedition, providing a eukaryotic community dataset linked to microplastic-associated samples across global ocean transects. The dataset is a duplicate record of the same Tara Mission Microplastics 18S V9 ASV table, including contextual data and phyloseq-formatted outputs.
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