We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Evaluating the potential of marine invertebrate and insect protein hydrolysates to reduce fetal bovine serum in cell culture media for cultivated fish production
Summary
Researchers found that protein hydrolysates from black soldier fly, cricket, oyster, mussel, and lugworm at low concentrations can significantly enhance zebrafish embryonic stem cell growth in culture media containing 50% less fetal bovine serum, pointing toward more sustainable and cost-effective cell culture alternatives.
Abstract The use of fetal bovine serum (FBS) and the price of the cell culture media are the key constraints for developing serum-free cost-effective media. This study aims to replace or reduce the typical 10% serum application in fish cell culture media by applying protein hydrolysates from insects and marine invertebrate species for the growth of Zebrafish embryonic stem cells (ESC) as the model organism. Protein hydrolysates were produced from Black soldier fly (BSF), cricket, oyster, mussel, and lugworm with high protein content, suitable functional properties, adequate amino acids composition, and the degree of hydrolysis from 18.24 to 33.52%. Protein hydrolysates at low concentrations from 0.001 to 0.1 mg/mL in combination with 1 and 2.5% serum significantly increased cell growth compared to the control groups (5 and 10% serum) (P < 0.05). All protein hydrolysates with concentrations of 1 and 10 mg/mL were found to be toxic to cells and significantly reduced cell growth and performance (P < 0.05). However, except for cricket, all hydrolysates were able to restore or significantly increase cell growth and viability with 50% less serum at a concentration of 0.001, 0.01, and 0.1 mg/mL. Although cell growth was enhanced at lower concentrations of protein hydrolysates, cell morphology was altered due to the lack of serum. Lactate dehydrogenase (LDH) activity results indicated that BSF and lugworm hydrolysates did not alter the cell membrane. In addition, light and fluorescence imaging revealed that cell morphological features were comparable to the 10% serum control group. Overall, lugworm and BSF hydrolysates reduced serum by up to 90% while preserving excellent cell health.
Sign in to start a discussion.
More Papers Like This
Evaluating the Potential of Marine Invertebrate and Insect Protein Hydrolysates to Reduce Fetal Bovine Serum in Cell Culture Media for Cultivated Fish Production
Researchers evaluated protein hydrolysates from insects and marine invertebrates as replacements for fetal bovine serum in fish cell culture media, working toward more sustainable and cost-effective approaches for cultivated fish production.
Sustainable Alternative Feed for Aquaculture: State of the Art and Future Perspective
Not directly relevant to microplastics — this review surveys sustainable alternative feed ingredients for aquaculture, covering plant-based proteins, by-products, and insects as replacements for fishmeal and fish oil.
Supplementation of Enzymatic Hydrolysate in Low-Fishmeal and Low-Crop Diet Improves Growth, Antioxidant Capacity, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus (Selenka)
Researchers tested whether enzymatic hydrolysate could replace fishmeal and soybean meal in diets for juvenile sea cucumbers, an important aquaculture species. They found that sea cucumbers fed the hydrolysate-supplemented diet showed improved growth, stronger antioxidant defenses, and enhanced immune function. The study supports using enzymatic hydrolysate as a sustainable feed ingredient that could reduce aquaculture's dependence on conventional protein sources.
Combined Effects of Yellow Mealworm (Tenebrio molitor) and Saccharomyces cerevisiae on the Growth Performance, Feed Utilization Intestinal Health, and Blood Biomarkers of Nile Tilapia (Oreochromis niloticus) Fed Fish Meal-Free Diets
Researchers tested yellow mealworm meal as a replacement for fishmeal in Nile tilapia diets, finding that adding the probiotic yeast Saccharomyces cerevisiae improved fish growth, gut health, liver condition, and immune function. This suggests insect-based feeds combined with probiotics can sustainably replace traditional fishmeal in aquaculture.
Reprocessing seafood waste: challenge to develop aquatic clean meat from fish cells
Researchers discovered that cells derived from discarded fish fins can naturally change shape into muscle-like and fat-like cells without genetic modification, successfully producing a prototype of lab-grown 'aquatic clean meat' and offering a sustainable, low-waste approach to future seafood production.