We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Evaluating the Potential of Marine Invertebrate and Insect Protein Hydrolysates to Reduce Fetal Bovine Serum in Cell Culture Media for Cultivated Fish Production
Summary
Researchers evaluated protein hydrolysates from insects and marine invertebrates as replacements for fetal bovine serum in fish cell culture media, working toward more sustainable and cost-effective approaches for cultivated fish production.
The use of fetal bovine serum (FBS) and the price of cell culture media are the key constraints for developing serum-free cost-effective media. This study aims to replace or reduce the typical 10% serum application in fish cell culture media by applying protein hydrolysates from insects and marine invertebrate species for the growth of Zebrafish embryonic stem cells (ESC) as the model organism. Protein hydrolysates were produced from black soldier flies (BSF), crickets, oysters, mussels, and lugworms with a high protein content, suitable functional properties, and adequate amino-acid composition, with the degree of hydrolysis from 18.24 to 33.52%. Protein hydrolysates at low concentrations from 0.001 to 0.1 mg/mL in combination with 1 and 2.5% serums significantly increased cell growth compared to the control groups (5 and 10% serums) (p < 0.05). All protein hydrolysates with concentrations of 1 and 10 mg/mL were found to be toxic to cells and significantly reduced cell growth and performance (p < 0.05). However, except for crickets, all the hydrolysates were able to restore or significantly increase cell growth and viability with 50% less serum at concentrations of 0.001, 0.01, and 0.1 mg/mL. Although cell growth was enhanced at lower concentrations of protein hydrolysates, the cell morphology was altered due to the lack of serum. The lactate dehydrogenase (LDH) activity results indicated that BSF and lugworm hydrolysates did not alter the cell membrane. In addition, light and fluorescence imaging revealed that the cell morphological features were comparable to those of the 10% serum control group. Overall, lugworm and BSF hydrolysates reduced the serum by up to 90% while preserving excellent cell health.
Sign in to start a discussion.
More Papers Like This
Evaluating the potential of marine invertebrate and insect protein hydrolysates to reduce fetal bovine serum in cell culture media for cultivated fish production
Researchers found that protein hydrolysates from black soldier fly, cricket, oyster, mussel, and lugworm at low concentrations can significantly enhance zebrafish embryonic stem cell growth in culture media containing 50% less fetal bovine serum, pointing toward more sustainable and cost-effective cell culture alternatives.
Sustainable Alternative Feed for Aquaculture: State of the Art and Future Perspective
Not directly relevant to microplastics — this review surveys sustainable alternative feed ingredients for aquaculture, covering plant-based proteins, by-products, and insects as replacements for fishmeal and fish oil.
Analysis of the Possibility of Feed Protein Additive Producing from Fish Scales
Researchers analyzed the chemical composition of bream fish scales, finding high concentrations of proteins and lipids including both saturated and unsaturated fatty acids, and assessed their potential as a protein feed additive for animals and aquaculture. The study establishes bream scales as a viable source of nutrients for feed production, offering a pathway for valorizing fish processing waste.
Cell-cultivated aquatic food products: emerging production systems for seafood
This review examines cell-cultivated seafood, a new approach to producing fish protein by growing fish cells in a lab rather than catching or farming fish. One potential benefit is avoiding the microplastic contamination found in wild and farmed fish, since the production environment can be controlled. As concerns grow about microplastics accumulating in seafood, lab-grown alternatives could offer a way to reduce human exposure to microplastics through diet.
Supplementation of Enzymatic Hydrolysate in Low-Fishmeal and Low-Crop Diet Improves Growth, Antioxidant Capacity, and Immunity of Juvenile Sea Cucumber Apostichopus japonicus (Selenka)
Researchers tested whether enzymatic hydrolysate could replace fishmeal and soybean meal in diets for juvenile sea cucumbers, an important aquaculture species. They found that sea cucumbers fed the hydrolysate-supplemented diet showed improved growth, stronger antioxidant defenses, and enhanced immune function. The study supports using enzymatic hydrolysate as a sustainable feed ingredient that could reduce aquaculture's dependence on conventional protein sources.