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Selective Fluorescence Staining of Microplastic in Water Utilizing Nile Red/surfactant Combination
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Korean researchers developed a method to selectively stain microplastics in water using a fluorescent dye combined with a surfactant, allowing microplastics to be detected without the usual filtering and separation steps. The technique could enable faster, simpler monitoring of microplastics directly in water samples.
본 연구에서는 염료와 계면활성제의 조합을 통해 미세플라스틱(microplastic, MP)을 수계 내에서 바로 선택적으로 형광 염색하는 방법을 도출하고자 하였다. 계면활성제의 존재 하에서 Nile red로 대표되는 비극성 형광 염료는 MP에 흡착되고 열처리에 의하여 고분자 매트릭스에 침투되어, MP의 안정적인 형광 거동을 제공하였다. 온도, 시간, 염료/계면활성제 비율, 염료/MP 비율, 그리고 MP 농도를 포함한 다양한 염색 변수의 영향을 조사하여 보다 바람직한 염색 조건을 찾고자 하였다. 또한, 아닐린의 투입에 의하여 수용액 내 비흡착 염료에 대한 형광 소광이 가능하였으며, 이를 통하여 MP의 수계 내 선택적 형광 염색을 성공적으로 달성하였다. 이 방법을 통하여, MP의 물로부터의 분리/여과 등 통상적인 절차를 생략하고, MP의 신속한 검출 및 모니터링을 위한 형광 염색이 가능해질 수 있을 것으로 기대된다.
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