We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Effects of diurnal temperature fluctuations on growth performance, energy metabolism, stress response, and gut microbes of juvenile mud crab Scylla paramamosain
Summary
A 51-day experiment with juvenile mud crabs Scylla paramamosain exposed to varying levels of daily temperature fluctuation found that larger fluctuations reduced survival, suppressed growth, disrupted energy metabolism, and altered gut microbiota composition. The results indicate that daily temperature variability, a key aspect of climate change, represents a significant physiological stressor for commercially important crustaceans.
A 51-day experiment was conducted to investigate the effects of diurnal temperature fluctuations (DTF) on growth performance, energy metabolism, stress response and gut microbiota of juvenile mud crab Scylla paramamosain . One control and three fluctuation groups were set up, i.e ., constant (28 ± 0°C) (CT), slight (SF) (28 ± 2°C), medium (MF) (28 ± 4°C), and large (LF) (28 ± 6°C) DTF. The survival rate tended to decrease with the intensification of DTF, the survival of rate of CT, SF, MF and LF were 80.6 ± 3.9%, 75.0 ± 6.8%, 33.3 ± 6.8%, and 30.6 ± 10.4% respectively. The crab in SF and MF had a shorter, but LF had a longer molt interval compared with the crab in the CT group. Cortisol, blood glucose (GLU), total cholesterol (T-CHO) and triglyceride (TG) levels peaked in the LF group. The exacerbation of DTF caused a dramatic increase in reactive oxygen species (ROS) levels and impacted the antioxidant capacity of juvenile crabs. The relative expression of AMP-activated protein kinase ( ampk ), heat shock protein 70 ( hsp70 ) and heat shock protein 90 ( hsp90 ) genes was significantly increased in MF group. The expression of Ribosomal protein S6 kinase ( s6k ) and Mechanistic target of rapamycin ( tor ) genes was significantly up-regulated in the SF group ( P< 0.05) but large DTF caused a decrease in the relative expression of a large number of functional genes. DTF affected the structure and function of gut microbes. The bacterial community changed with the intensification of DTF and alpha diversity continued to rise. Five biomarkers were identified, where Rhodobacterales and Rhodobacterac were significantly more abundant in the CT group, Campylobacterales,Vibrionales and erysipelotrichale s were more abundant in the SF, MF and LF groups. In addition, SF also enhanced gut microbes interactions compared with other treatments. These results suggest that drastic environmental DTF reduced the growth and survival of young mud crabs, and the effect was mediated by energy metabolism, antioxidant pathways and gut microbes.
Sign in to start a discussion.
More Papers Like This
Effects of different temperatures on growth and intestinal microbial composition of juvenile Eriocheir sinensis
Researchers examined how different water temperatures affect the growth and intestinal microbial composition of juvenile Chinese mitten crabs (Eriocheir sinensis), finding that temperature-driven changes in gut microbiota composition influence crab development.
Effects of temperature on the behaviour and metabolism of an intertidal foraminifera and consequences for benthic ecosystem functioning
Researchers exposed a common intertidal single-celled organism to heatwave-level temperatures and found it reduced activity by up to 80% and stopped burrowing entirely above 32°C, suggesting that climate-driven heatwaves could significantly disrupt sediment mixing and nutrient cycling in coastal mudflat ecosystems.
Warming-induced microplastic accumulation and physiological toxicity in fiddler crabs
Researchers studied the combined effects of microplastic exposure and rising water temperatures on fiddler crabs. They found that microplastics accumulated most heavily in the gills, and that warmer temperatures altered how the crabs responded to the plastic particles, increasing oxygen consumption and affecting antioxidant defenses. The study highlights how climate change and plastic pollution together may create compounding stress for coastal marine organisms.
The effects of microplastic ingestion and environmental warming on camouflage and growth in common shore crabs
Researchers found that shore crabs exposed to both microplastics and warming water showed impaired camouflage ability and reduced growth compared to crabs exposed to either stressor alone. The combined effects of microplastic pollution and climate change may be more harmful to marine life than either threat in isolation.
The gut microbial of sea urchin ( Strongylocentrotus intermedius ) under different temperatures: Microbial structure and co-occurrence patterns
Researchers exposed sea urchins to five temperatures ranging from 13 to 25°C and used high-throughput sequencing to show that elevated temperatures increase gut bacterial diversity, shift dominant genera, alter key metabolic pathways, and strengthen deterministic assembly processes, providing mechanistic insight into how warming reshapes invertebrate gut microbiomes.