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Disruption of hepatic metabolism in Lep KO mice.
Summary
Researchers found that polystyrene microplastics administered orally for nine weeks accumulated in liver tissue of leptin-knockout obese mice and induced histopathological liver alterations, including disruption of hepatic lipid, glucose, and amino acid metabolism.
(a) Experimental scheme. PS-MP was orally administrated into WT and Lep KO mice for 9 weeks. (b) Accumulation of MP in liver tissue. The red fluorescence of MP was detected under a fluorescence microscope at 100× and 400 × magnification. Arrows indicate MP. The frozen sections of liver tissues were prepared in triplicates of each mice, and each section was analyzed twice. (c) Histopathological structure of the liver. Pathological alterations were analyzed in H&E-stained liver sections at 100× and 400 × magnification. Arrow heads indicate microvesicular steatosis and arrows indicate macrovesicular steatosis. The H&E-stained sections of liver tissues were prepared in triplicates of each mice, and their histopathological factors were analyzed twice per each section. The data are reported as mean ± SD values. *, p < 0.05 compared to the Vehicle-treated group. #, p < 0.05 compared to the WT mice. Abbreviation: WT; wild type, Lep KO; leptin knockout, MP; microplastics, H&E; hematoxylin and eosin, CV; central vein.