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Isolation and identification of soil bacteria capable of degrading biodegradable mulch films

Biodegradation 2025 Score: 38 ? 0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Harshal J. Kansara, Yvan D. Hernandez-Charpak, André O. Hudson, Thomas A. Trabold, Jeffrey S. Lodge, Carlos Diaz

Summary

Researchers isolated and identified soil bacteria, including Pseudomonas guariconensis and Achromobacter species, capable of degrading biodegradable agricultural mulch films, demonstrating that culture-enrichment approaches can identify microbial candidates to accelerate the slow and inconsistent breakdown of biodegradable plastics in soil.

Polymers

Agricultural mulch films (AMFs) enhance crop productivity by controlling soil temperature and moisture and suppressing weed growth. Conventional AMFs made from polyethylene (PE) pose disposal challenges and contribute to long-term plastic pollution. Biodegradable mulch films (BMFs) offer a promising alternative, but their degradation in soil remains slow and inconsistent. This study employed a culture-enrichment approach to isolate soil bacteria (i.e., Pseudomonas guariconensis and Achromobacter denitrificans) capable of accelerating BMF biodegradation. Bioaugmentation with P. guariconensis enhanced CO₂ evolution in soil, with 48% and 36% carbon mineralization for two commercial BMFs (i.e., Bio360 and EcoVio), compared to 17% and 6.2% in non-inoculated soils. These findings demonstrate that targeted bacterial enrichment can accelerate BMF degradation, highlighting the potential for bioaugmentation to mitigate plastic accumulation in agricultural soils.

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