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Feasibility of diffuser-incorporated near-infrared trans-reflectance measurement for quantitative detection of microplastics captured in perfluorocarbon
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This study developed a near-infrared optical detection method using a perfluorocarbon carrier fluid to capture and quantify polyethylene microplastic particles, demonstrating good sensitivity and specificity. The approach offers a promising detection technique that could be adapted for monitoring microplastic contamination in water.
A diffuser-incorporated near-infrared (NIR) trans-reflectance measurement is demonstrated for quantitative detection of polyethylene (PE) particles captured in perfluorohexane (PFH, CF). PFH effectively captures PE particles through its hydrophobicity and absorbs little NIR radiation, recommending it for use in background-free NIR detection of captured PE particles. A reflective metal disk was used to push the captured PE particles in PFH toward the bottom of the vial that contained the sample, and the trans-reflectance measurement was performed by illuminating NIR radiation from the bottom of the vial at 45. Reproducibility is limited by the variation in the positions of small PE-particle aggregates at the water/PFH interface and the difficulty in ensuring full NIR sampling (coverage) of large aggregates. An effective way to secure improved reproducibility under these circumstances is illumination of broader and more uniform NIR radiation for measurement. For this purpose, a polytetrafluoroethylene (PTFE) disk was uniquely incorporated as a diffuser for the trans-reflectance measurement. Compared to the measurement with no diffuser, the diffuser-incorporated scheme produced more distinct PE peaks of low-quantity samples (0.1 and 0.2 mg) and enhanced the reproducibility in measurements of all the samples (0.1-4.0 mg of PE). As a result, the correlation between peak intensity and particle quantity was excellent (R: 0.997), and a limit of detection of 0.07 mg was achieved.
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