We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
[Occurrence Characteristics of Microplastics and Metal Elements in the Surface Water of Huangpu River and Their Associations with Metal Resistance Genes].
Summary
This Chinese study analyzed microplastic concentrations and metal elements in surface water samples from the Huangpu River, also examining their associations with metal resistance genes in microbial communities. Microplastics in urban rivers can carry both heavy metals and antibiotic resistance genes, amplifying public health risks.
Urban rivers have been regarded as the "hotspots" for microplastic (MPs) and metal contamination as they play important roles in pollution migration. However, as important sinks and sources of resistance genes, there has been little to no research investigating the associations between MPs, metal contaminations, and metal resistance genes (MRGs). Ten water samples were collected from the Huangpu River in situ; along with metal elements, MPs characteristics analyzed. Metal resistance genes and mobile genetic elements (MGEs) in waters and MPs were detected using metagenomic technology. As a result, the highest metal concentration was that of Sb in surface water (3.16±0.419) μg·L-1. The average abundance of MPs was (1.78±0.84) n·L-1, and the peak levels located in industrial and densely populated areas, which was significantly higher than those in agricultural and low population density areas. Fibrous, small-size (<0.5 mm), and transparent polyethylene terephthalate (PET) were the largest contributors of MPs. Eighteen MRGs were detected in all the samples. The relative abundance of MRGs in water was 1.68±0.21. The most dominant MRGs subtypes were merR and ruvB, which are subtypes resistant to mercury and Multi_metals. Correlation analysis showed that chromium and nickel in waters were significantly positively associated with MRG-Cr, MRG-Ni, and Multi_metals resistance genes. For MPs particles, the relative abundance of MRGs was 1.63±0.53. The most dominant MRGs subtypes were merT-P and copB, which also belong to mercury-resistant and Multi_metals. The Multi_metals resistance gene, ctpC, cueA, czrA, kmtR, etc., had significant positive associations with Ni, Cr, and Sb in waters. Compared with water samples, MPs selectively enriched merT-P, copB, ziaA, sodA, and dmeF. Additionally, the co-occurrence patterns of MRGs and MGEs were explored based on network analysis. In water samples, the transposases (tnpA_1 and tnpA_2), integrase (qacEdelta), and insertion sequence (IS91) were the major contributors of the horizontal gene transfer (HGT) of specific MRGs. Multiple subtypes resistant to copper and Multi_metals resistance genes on MPs were positively associated with IncFIC(FII), Rep7, rep7, and rep13, which were subtypes of plasmids. The presence of MPs exerted a significant impact on HGT of specific MRGs mediated by plasmids.
Sign in to start a discussion.
More Papers Like This
Evidence of selective enrichment of bacterial assemblages and antibiotic resistant genes by microplastics in urban rivers
Researchers sampled microplastics from two urban rivers in China and found that the bacterial communities colonizing plastic particles were distinctly different from those in the surrounding water. The microplastic-associated bacteria had lower diversity but higher proportions of biofilm-forming species and functions linked to human disease. Notably, the study found that microplastics selectively enriched antibiotic resistance genes, raising concerns about plastics serving as reservoirs for drug-resistant bacteria.
[Microplastics-Induced Shifts of Diversity and Abundance of Antibiotic Resistance Genes in River Water].
This Chinese study used high-throughput quantitative PCR to measure how different microplastic types affect the diversity and abundance of antibiotic resistance genes in river water. Polystyrene and polyethylene microplastics both increased the overall abundance of resistance genes, supporting concerns that microplastics act as reservoirs and spreaders of antibiotic resistance in freshwater systems.
Distinct profile of bacterial community and antibiotic resistance genes on microplastics in Ganjiang River at the watershed level
Researchers investigated microplastic pollution and associated bacterial communities, human pathogenic bacteria, and antibiotic resistance genes across the Ganjiang River watershed. They found microplastics were widely distributed with an average of 407 particles per cubic meter, and that microplastic surfaces harbored significantly higher bacterial diversity and more antibiotic resistance genes than surrounding water or sediment.
Metagenomic insights into ecological risk of antibiotic resistome and mobilome in riverine plastisphere under impact of urbanization
This study used advanced genetic sequencing to examine antibiotic resistance genes on microplastics found in an urban river. Microplastics harbored more antibiotic resistance genes and mobile genetic elements than natural materials like rocks and wood, and the problem was worse in more urbanized areas. The findings suggest that microplastics in waterways can act as hotspots for spreading antibiotic resistance, which is a growing public health threat.
Impact of Urbanization on Antibiotic Resistome in Different Microplastics: Evidence from a Large-Scale Whole River Analysis
Researchers conducted a large-scale river survey across urbanization gradients and characterized antibiotic resistance genes on microplastics from each zone, finding that urbanization level strongly predicted the diversity and abundance of resistance genes on plastic surfaces.