Editorial: Anaerobic digestion of waste organics: toxicity and management

As an important anaerobic environmental biotechnology, anaerobic digestion (AD) can convert biodegradable organic wastes, including organic wastewaters, waste activated sludge, and food wastes, into biogas (Dai et al., 2020; Toutian et al., 2020). AD involves cascading steps of hydrolysis/acidogenesis, acetogenesis/homoacetogensis, and methanogenesis, in which the distinct different microorganisms involved, including acidogenic bacteria, acetogens, homoacetogens, hydrogenotrophic and aceticlastic methanogens (Saha et al., 2020). This method offers several advantages, including low running cost, being adaptable to various feedstock or conditions, and not requiring sterilization. However, toxicants present in organic wastes, such as antibiotics, metal ions, nanoparticles, and microplastic fiber, may hinder the overall performance of AD (Hart et al., 2022; Mu and Chen, 2011; Tang et al., 2023). The present Research Topic “Anaerobic Digestion of Waste Organics: Toxicity and Management” aims to cover promising and novel researches into biological strategies to enhance AD performance, especially employing physicochemical analyses and multi-omics technologies. This topic comprises 4 original articles contributed by 23 authors. Propionic acid (HPr) is known as a frequently accumulated intermediate in anaerobic digesters due to the thermodynamical limitation. Thus, Kim et al. identify key players as metagenome-assembled genomes in HPr oxidation and organic overloading recovery in anaerobic digesters. The results showed that at least two key species of JABUEY01 sp013314815 and Methanoculleus sp002497965 are responsible for efficient propionate removal, which can be used as microbial cocktails for the stable operation of AD. The conductive materials of biochar and activated carbon were capable to enhance methane production from organic wastes via direct interspecific electron transfer (DIET) between acidogens and methanogens. Thus, Abid et al. investigated the impact of biochar on the anaerobic degradation of olive mill wastewater. The results demonstrated that adding biochar to olive mill wastewater increased the methane yield by 97.8%. The microbial diversity revealed that biochar supplementation significantly increased the abundance of potential genera of Methanothrix and Methanosarcina involved in DIET. Meanwhile, Wu et al. studied the effects of activated carbon- and graphite-conductive media on methane production in WAS fermentation. Their results show that the largest biogas yield in a 100 mesh-activated carbon group was 468.2 mL/g VSS, which was 13.8% higher than the blank group. Three genera of Nitrososphaeraceae, Methanobacterium, and Methanosaeta were cultured in the activated carbon and graphite groups. On the other hand, mitigating CH4 emissions may reduce the current global warming effects due to its high global warming potential. Thus, Im et al. reported that adding salt to the pig slurry storage tank can inhibit the activity of methanogens and reduce unwanted CH4 emissions. More CH4 can be obtained in the following biogas production from the stored pig slurry due to the preservation of organics. In summary, the articles included in this Research Topic demonstrate the importance of toxicity and management in the anaerobic digestion of waste organics. And the physicochemical analyses and potentially augmented by omics technologies are shown to be useful tools to provide solid evidence to the experiment results and reveal the potential mechanism. The outcome of such studies may improve the understanding of toxicity and management of organic wastes degradation and subsequently help enhance the development of anaerobic fermentation.