We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
The Phosphoproteomic Response of Pepper (Capsicum annuum L.) Seedlings to Selenium Stress
Summary
Researchers investigated the phosphoproteomic response of pepper (Capsicum annuum L.) seedlings to excess selenium stress using Tandem Mass Tag quantitative proteomics, identifying 4,434 phosphorylation sites on 2,058 proteins. They found that upregulated phosphoproteins were strongly associated with phenylpropanoid biosynthesis and nicotinamide metabolism pathways, while downregulated proteins were linked to ABC transporters and plant hormone signalling, providing new insights into plant selenium toxicity mechanisms.
Excessive selenium has gradually become a potential environmentally hazardous element for all organisms. Limited knowledge is available regarding the toxic mechanism of selenium in pepper, so the quantitative proteomics of phosphorylation was studied by Tandem Mass Tag approaches. A total of 4434 phosphorylation sites were identified on 2058 proteins, of which 3749 sites of 1919 proteins contained quantitative information. In the Se/mock (seedlings without Se treatment) comparison group, the number of upregulated phosphoproteins (658) was significantly higher than that of the downregulated ones (61). Systematic bioinformatics analysis, including protein annotation, functional classification, subcellular localization, and cluster analysis was performed. A total of 33 over-represented motifs were found in serine phosphorylation, and the most frequent motif was ‘sP’ (308 occurrences). According to KEGG enrichment analysis, the upregulated phosphorylated proteins (DPPs) were most strongly associated with the ’phenylpropanoid biosynthesis’ and ’nicotinate and nicotinamide metabolism’ pathways, while those that were downregulated were associated with the ’ABC transporters’ and ‘plant hormone signal transduction’ pathways. Our data can provide new insights for evaluating the response mechanism of plants to selenium pollution and improving their resistance to selenium.
Sign in to start a discussion.
More Papers Like This
Integrative Physiological and Transcriptome Analysis Reveals the Mechanism of Cd Tolerance in Sinapis alba
This paper is not about microplastics; it uses transcriptomics and physiological measurements to understand how white mustard (Sinapis alba) tolerates cadmium heavy metal stress at the molecular level.
Modulations in protein phosphorylation explain the physiological responses of barley (Hordeum vulgare) to nanoplastics and ZnO nanoparticles
Researchers examined how the co-exposure of barley plants to nanoplastics and zinc oxide nanoparticles affects protein phosphorylation and physiological responses. The study found that the combination of nanomaterials increased oxidative stress and altered hormone levels more than individual exposures, suggesting that interactions between nanoplastics and other nanomaterials in the environment may amplify their effects on plant health.
Ultrastructural and Proteomic Analyses Revealed the Mechanism by Which Foliar Spraying of Se Nanoparticles Alleviated the Toxicity of Microplastics in Pistia stratiotes L.
Foliar application of selenium nanoparticles to the aquatic plant Pistia stratiotes alleviated toxicity from polyethylene nanoplastics, with ultrastructural and proteomic analyses revealing that selenium nanoparticles protected photosynthetic machinery and antioxidant systems.
Unraveling the Complex Physiological, Biochemical, and Transcriptomic Responses of Pea Sprouts to Salinity Stress
Researchers investigated the physiological, biochemical, and transcriptomic responses of pea sprouts to high salinity stress, analyzing the ascorbic acid-glutathione cycle, endogenous hormone levels, metabolite profiles, and gene expression patterns. The study revealed coordinated redox-metabolic adjustments and transcriptome reprogramming that mediate ionic stress tolerance in this nutrient-rich crop.
Protective role of nano-selenium on Gymnocypris przewalskii under saline–alkaline stress: a comprehensive analysis of transcriptomics and metabolomics
Scientists studied a type of fish that lives in very salty, harsh water and found that tiny selenium particles helped protect the fish from stress and damage. The selenium particles worked by changing how the fish's genes and body chemistry responded to the difficult environment. While this study was done in fish, selenium is an important nutrient for humans too, and this research could help scientists better understand how selenium protects our bodies from environmental stress and damage.