We can't find the internet
Attempting to reconnect
Something went wrong!
Hang in there while we get back on track
Integrative Physiological and Transcriptome Analysis Reveals the Mechanism of Cd Tolerance in Sinapis alba
Summary
This paper is not about microplastics; it uses transcriptomics and physiological measurements to understand how white mustard (Sinapis alba) tolerates cadmium heavy metal stress at the molecular level.
Recently, pollution caused by the heavy metal Cd has seriously affected the environment and agricultural crops. While Sinapis alba is known for its edible and medicinal value, its tolerance to Cd and molecular response mechanism remain unknown. This study aimed to analyze the tolerance of S. alba to Cd and investigate its molecular response mechanism through transcriptomic and physiological indicators. To achieve this, S. alba seedlings were treated with different concentrations of CdCl2 (0.25 mmol/L, 0.5 mmol/L, and 1.0 mmol/L) for three days. Based on seedling performance, S. alba exhibited some tolerance to a low concentration of Cd stress (0.25 mmol/L CdCl2) and a strong Cd accumulation ability in its roots. The activities and contents of several antioxidant enzymes generally exhibited an increase under the treatment of 0.25 mmol/L CdCl2 but decreased under the treatment of higher CdCl2 concentrations. In particular, the proline (Pro) content was extremely elevated under the 0.25 and 0.5 mmol/L CdCl2 treatments but sharply declined under the 1.0 mmol/L CdCl2 treatment, suggesting that Pro is involved in the tolerance of S. alba to low concentration of Cd stress. In addition, RNA sequencing was utilized to analyze the gene expression profiles of S. alba exposed to Cd (under the treatment of 0.25 mmol/L CdCl2). The results indicate that roots were more susceptible to disturbance from Cd stress, as evidenced by the detection of 542 differentially expressed genes (DEGs) in roots compared to only 37 DEGs in leaves. GO and KEGG analyses found that the DEGs induced by Cd stress were primarily enriched in metabolic pathways, plant hormone signal transduction, and the biosynthesis of secondary metabolites. The key pathway hub genes were mainly associated with intracellular ion transport and cell wall synthesis. These findings suggest that S. alba is tolerant to a degree of Cd stress, but is also susceptible to the toxic effects of Cd. Furthermore, these results provide a theoretical basis for understanding Cd tolerance in S. alba.
Sign in to start a discussion.
More Papers Like This
Transcriptomic and Functional Analyses of Two Cadmium Hyper-Enriched Duckweed Strains Reveal Putative Cadmium Tolerance Mechanisms
Not directly relevant to microplastics — this study uses transcriptomics to investigate how duckweed tolerates and accumulates cadmium, exploring potential mechanisms for heavy-metal phytoremediation.
Nutrient Metabolism Pathways Analysis and Key Candidate Genes Identification Corresponding to Cadmium Stress in Buckwheat through Multiomics Analysis
This study used transcriptomic and metabolomic analysis to investigate how buckwheat responds to cadmium stress at the molecular level, identifying key metabolic pathways affected by the heavy metal. It is not about microplastics and is not relevant to microplastic research.
Assessing stress responses in potherb mustard (Brassica juncea var. multiceps) exposed to a synergy of microplastics and cadmium: Insights from physiology, oxidative damage, and metabolomics
Researchers found that microplastics in soil increased the amount of cadmium, a toxic heavy metal, that mustard green plants absorbed, while also reducing crop yields and photosynthesis. Higher concentrations of microplastics made more cadmium available in the soil, leading to greater accumulation of the metal in the plants. This raises food safety concerns because vegetables grown in microplastic-contaminated soil could contain higher levels of toxic metals that are harmful to human health.
Arabidopsis Transcription Factor WRKY45 Confers Cadmium Tolerance via Activating PCS1 and PCS2 Expression
Not relevant to microplastics — this study investigates how the plant transcription factor WRKY45 helps Arabidopsis tolerate cadmium heavy metal stress by activating genes for detoxifying compounds.
Physiological and Cellular Ultrastructural Responses of Sesuvium portulacastrum under Cd Stress Grown Hydroponically
Researchers tested how the salt-tolerant plant Sesuvium portulacastrum responds to increasing concentrations of cadmium, a toxic heavy metal. The plant showed strong antioxidant defense mechanisms at lower cadmium levels but experienced significant cellular damage at higher concentrations, including disruption to chloroplasts and mitochondria. The findings suggest this species has moderate tolerance to heavy metal stress, which could make it useful for cleaning up contaminated environments.