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Exosomal miRNA analysis provides new insights into exposure to nanoplastics and okadaic acid
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Researchers extracted exosomes from human gastric cells (AGS) previously exposed to polystyrene nanoplastics and okadaic acid, profiling exosomal miRNA to gain new insights into combined toxicity mechanisms. The miRNA analysis revealed stress response pathways not detected in standard cytotoxicity assays, suggesting exosomal miRNA is a sensitive biomarker for nanoplastic and marine toxin exposure.
As an emerging environmental pollutant, nanoplastics (NPs) have attracted wide attention in terms of their impact on the ecological environment and human health. Currently, researches on the cytotoxicity of NPs mainly focus on oxidative stress, damage to the cell membrane and organelles, induction of immune response and genotoxicity. Okadaic acid (OA) is the main component of diarrheal shellfish toxin. Based on the previous combined toxicity exploration of polystyrene (PS) NPs and (OA) to human gastric adenocarcinoma (AGS) cells, cell-derived exosomes were extracted and exosomal miRNA profiles were analyzed for the first time in this study. The results showed that the composition of miRNAs varied after the exposure of NPs and OA. Specifically, the expression of miR-1-3p in both PS-Exo and PS-OA-Exo was significantly reduced. And the expression of miR-1248 was upregulated most significantly by comparing the DE miRNAs between PS-Exo and PS-OA-Exo. MiR-1-3p and miR-1248 may be the key genes for the combined toxicity of NPs and OA. After analysis, we found that both the decreased expression of miR-1-3p and the increased expression of miR-1248 can increase the expression of FN1 and affect DNA replication, which was surprisingly consistent with the results of our previous cytotoxicity studies. Since exosomal miRNAs are selectively encapsulated by donor cell, we speculate that the changes of exosomal miRNAs may due to the synchronous changes of intracellular environment and the downregulation of intracellular FN1 may be attributed to decreased expression of miR-1-3p and increased expression of miR-1248 in donor cells. Accordingly, we come to the conclusion that the changes of miRNAs in the exosomes derived from AGS cells after environmental stimulation could reflect the biological effects of donor cells.
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Polystyrene nanoplastics and the marine algal toxin okadaic acid were tested in combination against human gastric adenocarcinoma (AGS) cells to determine whether nanoplastics aggravate okadaic acid toxicity. PS nanoplastics enhanced toxicity by increasing reactive oxygen species production, inducing DNA damage, and impairing DNA replication and repair through the Fanconi anemia pathway.
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