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Application of fluorescent microscopy and Fourier-transform infrared spectroscopy for analysis of microplastics in Can Gio seawater
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Researchers validated a dual-method approach combining Nile Red fluorescence microscopy with infrared spectroscopy to detect microplastics in seawater, achieving recoveries of 88-97% across five plastic types. Applying the method to 27 samples from Can Gio Sea near Ho Chi Minh City, Vietnam, they found polyethylene and PET were the most abundant plastics — contributing new data from an understudied coastal region of Southeast Asia.
Microplastics (MPs) pollution has become a global challenge due to their persistent properties and harm to nature and human health. Observation of MPs pollution, especially in marine environments, is a critical concern in environmental analysis. In this work, we validate the method for detecting the presence of MPs in seawater by using fluorescence microscopy with Nile Red dye combined with Attenuated Total Reflectance Fourier-transform infrared spectroscopy (ATC-FTIR). The specificity and accuracy of this method were evaluated by analyzing the standard spiked samples. Five standard MPs and chitin samples used in this study were polyamide (PA), polyethylene (PE), polyethylene terephthalate (PET), poly(methyl methacrylate) (PMMA), and polyvinyl chloride (PVC) with a diameter from 5 to 300µm. The obtained recovery was 88.3-96.7%, with the related standard deviation (RSD%) in the range of 4.6-11.0%. This method was applied to determine microplastics in 27 seawater samples at three locations in Can Gio Sea, Ho Chi Minh City. The results showed the pollution level of each type of MP was significantly varied, with PE and PET being the highest, while there was almost no PMMA in seawater samples. ® 2023 Journal of Science and Technology - NTTU
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Application of fluorescent microscopy and Fourier-transform infrared spectroscopy for analysis of microplastics in Can Gio seawater
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Researchers validated the combined use of fluorescent microscopy and Fourier-transform infrared spectroscopy for detecting and identifying microplastics in marine environmental samples. The dual-technique approach improved detection sensitivity and chemical identification accuracy compared to either method used alone.
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Researchers used Raman microscopy to trace microplastics from Ho Chi Minh City urban canals through the Can Gio Mangrove Reserve to the East Sea, detecting common polymers including PE, PET, PA, PP, PVC, PS, and PMMA throughout. The study demonstrates how urban plastic waste can travel from inland waterways into coastal and marine ecosystems in Vietnam.
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Researchers assessed the spatiotemporal distribution of microplastics in estuaries and beaches of the Can Gio Biosphere Reserve in Ho Chi Minh City, Vietnam, collecting over 300 water and sediment samples across dry and rainy seasons and characterising particles by size, morphology, and polymer composition using FTIR spectroscopy.
Assessing Microplastic Prevalence and Dispersion from Saigon Urban Canals via Can Gio Mangrove Reserve to East Sea by Raman Scattering Microscopy
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Researchers used Raman scattering microscopy to assess microplastic prevalence and dispersion along a transect from Saigon urban canals through the Can Gio Mangrove Reserve to the East Sea, characterizing how urban plastic pollution travels through mangrove ecosystems into coastal marine waters.
Assessing microplastic dispersion from Saigon urban canals via Can Gio Mangrove Reserve to East Sea in Vietnam by Raman scattering microscopy
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This study traced the movement of microplastics from urban canals in Ho Chi Minh City through the Can Gio Mangrove Reserve to the East Sea using Raman scattering microscopy. Results confirmed that urban plastic pollution is transported through mangrove-lined coastal waterways to the open ocean.
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