Evaluation of Minimum Inhibitory Concentration of Heavy Metals Contained in Packaging Material Digest on Prominent Gut Microbiota

Several scientific investigations have revealed that the leaching of metals from packaging material into the packed food is an unavoidable process. Hence, this study is aimed at investigating the effect of leached heavy metals from food packing materials on normal human gut flora. We analysed the effect of vanadium, arsenic, cadmium, and mercury present in digested packaging materials (DPM) on standard strains of <i>Escherichia coli</i> ATCC 25923, <i>Pseudomonas aeruginosa</i> ATCC 27853, <i>Klebsiella pneumoniae</i> ATCC 70063, and <i>Enterococcus faecalis</i> ATCC 29212. The minimum inhibitory concentration (MIC) of laboratory-grade heavy metal salts and heavy metals present in DPM was determined by the agar dilution method. For all four bacteria, the MIC of cadmium and arsenic in the DPM was 7 <i>μ</i>g/ml and 1.6 <i>μ</i>g/ml, respectively. The MIC of mercury in DPM was 1.6 <i>μ</i>g/ml for <i>E. coli</i>, <i>K. pneumoniae</i>, and <i>E. faecalis</i> and 1.4 <i>μ</i>g/ml for <i>P. aeruginosa</i>. MIC of vanadium for <i>E. coli</i>, <i>P. aeruginosa</i>, and <i>E. faecalis</i> was 2.2 <i>μ</i>g/ml, and for <i>K. pneumoniae</i> was 2.0 <i>μ</i>g/ml. The difference in MICs of heavy metals in DPMs and heavy metal salts was not statistically significant. MICs were within CODEX-specified permissible levels. Though heavy metals in packaging material have not shown a deleterious effect on representative human gut flora, there is scope to study their effect on the gut microbiome. Thus, understanding the risk of heavy metal ingestion through unknown sources and avoiding any possible ingestion is crucial to preventing chronic diseases.