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Original research — experimental, observational, or case-control study. Direct primary evidence.
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Assessment of polystyrene nano plastics effect on human salivary α-amylase structural alteration: Insights from an in vitro and in silico study
International Journal of Biological Macromolecules2023
9 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 50
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0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Ananthaselvam Azhagesan,
Ananthaselvam Azhagesan,
Ananthaselvam Azhagesan,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Rinku Polachirakkal Varghese,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
Durgalakshmi Rajendran,
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
C. George Priya Doss,
Natarajan Chandrasekaran
Rinku Polachirakkal Varghese,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
Natarajan Chandrasekaran
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
C. George Priya Doss,
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
C. George Priya Doss,
C. George Priya Doss,
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
C. George Priya Doss,
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Natarajan Chandrasekaran
Summary
Researchers investigated how polystyrene nanoplastics interact with human salivary alpha-amylase, a key digestive enzyme, using both laboratory experiments and computer modeling. They found that the nanoplastics competitively inhibited the enzyme and caused structural changes including loss of secondary protein structure. The study suggests that nanoplastic exposure in the digestive system may interfere with normal enzyme function, raising concerns about potential impacts on human digestion.
The study found that the enzyme activity of human salivary α-amylase (α-AHS) was competitively inhibited by nanoplastic polystyrene (PS-NPs), with a half-inhibitory concentration (IC) of 92 μg/mL, while the maximum reaction rate (V) remained unchanged at 909 μg/mL•min. An increase in the concentration of PS-NPs led to a quenching of α-AHS fluorescence with a slight red shift, indicating a static mechanism. The binding constant (K) and quenching constant (K) were calculated to be 2.92 × 10 M and 1.078 × 10 M• S respectively, with a hill coefficient (n) close to one and an apparent binding equilibrium constant (K) of 1.54 × 10 M. Molecular docking results suggested that the interaction between α-AHS and PS-NPs involved π-anion interactions between the active site Asp197, Asp300 residues, and van der Waals force interactions affecting the Tyr, Trp, and other residues. Fourier transform infrared (FT-IR) and circular dichroism (CD) analyses revealed conformational changes in α-AHS, including a loss of secondary structure α-helix and β-sheet. The study concludes that the interaction between α-AHS and PS-NPs leads to structural and functional changes in α-AHS, potentially impacting human health. This research provides a foundation for further toxicological analysis of MPs/NPs in the human digestive system.