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Human Health Effects
Nanoplastics
Remediation
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Harmful effects of true-to-life nanoplastics derived from PET water bottles in human alveolar macrophages.
Environmental Pollution2024
21 citations
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Citation count from OpenAlex, updated daily. May differ slightly from the publisher's own count.
Score: 65
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0–100 AI score estimating relevance to the microplastics field. Papers below 30 are filtered from public browse.
Michelle Morataya-Reyes,
Ricard Marcos,
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Balasubramanyam Annangi,
Balasubramanyam Annangi,
Alireza Tavakolpournegari,
Alireza Tavakolpournegari,
Alireza Tavakolpournegari,
Jéssica Arribas Arranz,
Gooya Banaei,
Gooya Banaei,
Michelle Morataya-Reyes,
Aliro Villacorta
Alba Hernández,
Alba Hernández,
Aliro Villacorta
Balasubramanyam Annangi,
Susana Pastor,
Michelle Morataya-Reyes,
Ricard Marcos,
Ricard Marcos,
Balasubramanyam Annangi,
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Alba Hernández,
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Aliro Villacorta
Alba Hernández,
Aliro Villacorta
Aliro Villacorta
Alba Hernández,
Alba Hernández,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Ricard Marcos,
Michelle Morataya-Reyes,
Michelle Morataya-Reyes,
Michelle Morataya-Reyes,
Gooya Banaei,
Jéssica Arribas Arranz,
Jéssica Arribas Arranz,
Alireza Tavakolpournegari,
Alireza Tavakolpournegari,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Antonia Velázquez,
Jéssica Arribas Arranz,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Jéssica Arribas Arranz,
Ricard Marcos,
Jéssica Arribas Arranz,
Susana Pastor,
Jéssica Arribas Arranz,
Michelle Morataya-Reyes,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Alireza Tavakolpournegari,
Alba Hernández,
Gooya Banaei,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Susana Pastor,
Michelle Morataya-Reyes,
Aliro Villacorta
Alba Hernández,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Ricard Marcos,
Aliro Villacorta
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Susana Pastor,
Aliro Villacorta
Gooya Banaei,
Gooya Banaei,
Gooya Banaei,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alireza Tavakolpournegari,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Susana Pastor,
Susana Pastor,
Susana Pastor,
Susana Pastor,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Michelle Morataya-Reyes,
Antonia Velázquez,
Antonia Velázquez,
Ricard Marcos,
Antonia Velázquez,
Antonia Velázquez,
Ricard Marcos,
Aliro Villacorta
Ricard Marcos,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Alba Hernández,
Susana Pastor,
Alba Hernández,
Alba Hernández,
Ricard Marcos,
Ricard Marcos,
Ricard Marcos,
Alba Hernández,
Antonia Velázquez,
Alba Hernández,
Balasubramanyam Annangi,
Balasubramanyam Annangi,
Antonia Velázquez,
Alba Hernández,
Alba Hernández,
Jéssica Arribas Arranz,
Ricard Marcos,
Ricard Marcos,
Susana Pastor,
Susana Pastor,
Alba Hernández,
Alba Hernández,
Aliro Villacorta
Summary
Researchers tested nanoplastics derived from actual PET water bottles on mouse lung immune cells, focusing specifically on cells that had internalized the particles. Even though the nanoplastics were taken up by 100% of cells at the highest dose, they did not cause outright cell death. However, they did trigger significant increases in reactive oxygen species and shifted the immune cells toward a pro-inflammatory state, suggesting that inhaled nanoplastics from everyday plastic products could promote chronic lung inflammation.
The increasing presence of secondary micro/nanoplastics (MNPLs) in the environment requires knowing if they represent a real health concern. To such end, an important point is to test representative MNPLs such as the denominated true-to-life MNPLs, resulting from the degradation of plastic goods in lab conditions. In this study, we have used polyethylene terephthalate (PET) NPLs resulting from the degradation of PET water bottles. Since inhalation is an important exposure route to environmental MNPLS, we have used mouse alveolar macrophages (MH-S) as a target cell, and the study focused only on the cells that have internalized them. This type of approach is novel as it may capture the realistic adverse effects of PETNPLs only in the internalized cells, thereby mitigating any biases while assessing the risk of these MNPLs. Furthermore, the study utilized a set of biomarkers including intracellular reactive oxygen species (ROS) levels, variations on the mitochondrial membrane potential values, and the macrophage polarization to M1 (pro-inflammatory response) and M2 (anti-proinflammatory response) as possible cellular effects due to PETNPLs in only the cells that internalized PETNPLs. After exposures lasting for 3 and 24 h to a range of concentrations (0, 25, 50, and 100 μg/mL) the results indicate that no toxicity was induced despite the 100% internalization observed at the highest concentration. Significant intracellular levels of ROS were observed, mainly at exposures lasting for 24 h, in an indirect concentration-effect relationship. Interestingly, a reduction in the mitochondrial membrane potential was observed, but only at exposures lasting for 24 h, but without a clear concentration-effect relationship. Finally, PETNPL exposure shows a significant polarization from M0 to M1 and M2 subtypes. Polarization to M1 (pro-inflammatory stage) was more marked and occurred at both exposure times. Polarization to M2 (anti-inflammatory stage) was only observed after exposures lasting for 24 h. Due to the relevance of the described biomarkers, our results underscore the need for further research, to better understand the health implications associated with MNPL exposure.